The war against cancer: Suicide gene therapy

Muzeyyen Izmirli; Dilara Sonmez; Bulent Gogebakan

doi:10.18282/amor.v2.i3.103

The war against cancer: Suicide gene therapy

Abstract - 5107 PDF - 948

Muzeyyen Izmirli, Dilara Sonmez, Bulent Gogebakan

Abstract

The National Cancer Institute and the American Cancer Society announced that 1.6 million new cancer cases are projected to occur in the USA in 2016. One of the most innovative approaches against cancer is suicide gene therapy, in which suicide-inducing transgenes are introduced into cancer cells. When cancer treatments target the total elimination of tumor cells, there will be no side effects for normal cells. Cancer tissues are targeted through various targeted transport methods, followed by tissue-specific enzymes converting a systemically suitable prodrug into an active drug in the tumor. Suicidal genes are delivered by transporters, such as viral and non-viral vectors, into cancer cells. Suicide gene therapeutic strategies currently pursued are herpes simplex virus thymidine kinase gene with prodrug ganciclovir, cytosine deaminase gene, carboxyl esterase/irinotecan, varicella zoster virus thymidine kinase/6-methoxypurine arabinonucleoside, nitroreductase Nfsb/5-(aziridin-1-yl)-2,4-dinitrobenzamide, carboxypeptidase G2/4-[(2-chloroethyl)(2- mesyloxyethyl)amino]benzoyl-L-glutamic acid, cytochrome p450-isofosfamide, and cytochrome p450-cyclophosphamide. The goal of this review is to summarize the different suicide gene systems and gene delivery vectors addressed to cancer cells, with a particular emphasis on recently developed systems. Finally, we briefly describe the advantageous clinical applications and potential side effects of suicide gene therapy.

Keywords

suicide gene therapy; cancer; vector

Full Text:

PDF

References

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA Cancer J Clin 2016; 66(1): 7–30. doi: 10.3322/caa c.21332.

Wirth T, Parker N, Ylä-Herttuala S. History of gene therapy. Gene 2013; 525(2): 162–169. doi: 10.1016/j.gen e.2013.03.137.

Biasco L, Baricordi C, Aiuti A. Retroviral integrations in gene therapy trials. Mol Ther 2012; 20(4): 709–716. doi: 1 0.1038/mt.2011.289.

Duarte S, Carle G, Faneca H, Pedroso de Lima MC, Pierrefite-Carle V. Suicide gene therapy in cancer: Where do we stand now? Cancer Lett 2012; 324(2): 160–170. doi: 10.1016/j.canlet.2012.05.023.

Curigliano G, Cardinale D, Suter T, Palataniotis G, de Azambuja E, Sandri MT, Criscitiello C, Goldhirsch A, Cipolla C, Roila F. Cardiovascular toxicity induced by chemotherapy, targetedagents and radiotherapy: ESMO Clinical Practice Guidelines. Ann Oncol 2012; 23(7): 155–166. doi: 10.1093/annonc/mds293.

Malecki M. Frontiers in suicide gene therapy of cancer. J Genet Syndr Gene Ther 2012; 3(4): 1000119. doi: 10.417 2/2157-7412.1000119.

Zarogoulidis P, Darwiche K, Sakkas A, Yarmus L, Huang H, Li Q, Freitag L, Zarogoulidis K, Malecki M. Suicide gene therapy for cancer; Old dog new tricks?. J Genet Syndr Gene Ther 2013; 4(4): 1000139.doi: 10.4172/2157-7412.1000139.

Balfour HH Jr. Antiviral drugs. N Engl J Med 1999; 340(16): 1255–1268. doi: 10.1056/NEJM1999042234016 08.

Dong JY, Woraratanadharm J. Gene therapy vector design strategies for the treatment of cancer. Future Oncol 2005; 1(3): 361–373. doi: 10.1517/14796694.1.3.361.

Sancar A. Mechanisms of DNA excision repair. Science 1994; 266(5193): 1954–1956. doi: 10.1126/science.78011 20.

Wei SJ, Chao Y, Hung YM, Lin WC, Yang DM, Shih YL, Ch’ang LY, Whang-Pheng J, Yang WK. S- and G2-phase cell cycle arrests and apoptosis induced by ganciclovir in murine melanoma cells transduced with herpes simplex virus thymidine kinase. Exp Cell Res 1998; 241(1): 66–75.doi: 10.1006/excr.1998.4005.

Fischer U, Steffens S, Frank S, Rainov NG, Schulze-Osthoff K, Kramm CM. Mechanisms of thymidine kinase/ganciclovir and cytosine deaminase/5-fluorocytosine suicide gene therapy-induced cell death in glioma cells. Onco-gene 2005; 24(7): 1231–1243. doi: 10.1038/sj.onc.1208290.

Bondanza A, Hambach L, Aghai Z, Nijmeijer B, Kaneko S, Mastaglio S, Radrizzani M, Fleischauer K, Ciceri F, Bordignon C, Bonini C, Goulmy E. IL-7 receptor expression identifies suicide gene-modified allospecific CD8+ T cells capable of self-renewal and differentiation into antileukemia effectors. Blood 2011; 117(24): 6469–6478. doi: 10.1182/blood-2010-11-320366.

Tang W, He Y, Zhou S, Ma Y, Liu G. A novel Bifidobacterium infantis-mediated TK/GCV suicide gene therapy system exhibits antitumor activity in a rat model of bladder cancer. J Exp Clin Cancer Res 2009; 28: 155. doi: 10.1186/1756-9966-28-155.

Kakinoki K, Nakamoto Y, Kagaya T, Tsuchiyama T, Sakai Y, Nakahama T, Mukaida N, Kaneko S. Prevention of intrahepatic metastasis of liver cancer by suicide gene therapy and chemokine ligand 2/monocyte chemoattractant protein-1 delivery in mice. J Gene Med 2010; 12(12): 1002–1013. doi: 10.1002/jgm.1528.

Nasu Y, Saika T, Ebara S, Kusaka N, Kaku H, Abarzua F, Manabe D, Thompson TC, Kumon H. Suicide gene therapy with adenoviral delivery of HSV-tK gene for patients with local recurrence of prostate cancer after hormonal therapy. Mol Ther 2007; 15(4): 834–840. doi: 10.1038/sj.mt.6300096.

Negroni L, Samson M, Guigonis JM, Rossi B, Pierrefite-Carle V, Baudoin C. Treatment of colon cancer cells using the cytosine deaminase/5-fluorocytosine suicide system induces apoptosis, modulation of the proteome, and Hsp 90β phosphorylation. Mol Cancer Ther 2007; 6(10): 2747–2756. doi: 10.1158/1535-7163.MCT-07-0040.

Kanai F, Kawakami T, Hamada H, Sadata A, Yoshida Y, Tanaka T, Ohashi M, Tateishi K, Shiratori Y, Omata M. Adenovirus-mediated transduction of Escherichia coli uracil phosphoribosyltransferase gene sensitizes cancer cells to low concentrations of 5-fluorouracil. Cancer Res 1998; 58(9): 1946–1951.

Richard C, Duivenvoorden W, Bourbeau D, Massie B, Roa W, Yau J, Th’ng J. Sensitivity of 5-fluorouracil-resistant cancer cells to adenovirus suicide gene therapy. Cancer Gene Ther 2007; 14(1): 57–65. doi: 10.1038/sj.cgt.7700980.

Mullen CA, Coale MM, Lowe R, Blaese RM. Tumors expressing the cytosine deaminase suicidegene can be eliminated in vivo with 5-fluorocytosine and induce protective immunity to wild type tumor. Cancer Res 1994; 54(6): 1503–1506.

Ichikawa T, Tamiya T, Adachi Y, Ono Y, Matsumoto K, Furuta T, Yoshida Y, Hamada H, Ohmoto T. In vivo efficacy and toxicity of 5-fluorocytosine/cytosine deaminase gene therapy for malignant gliomas mediated by adenovirus. Cancer Gene Ther 2000; 7(1): 74–82. doi: 10.1038/sj.cgt.7700086.

Pandha HS, Martin LA, Rigg A, Hurst SC, Stamp GW, Sikora K, Lemoine R. Genetic prodrug activation therapy for breast cancer: A phase I clinical trial of erbB-2-directed suicide gene expression. J Clin Oncol 1999; 17(7): 2180–2189.

Freytag SO, Stricker H, Pegg J, Paielli D, Pradhan DG, Peabody J, DePeralta-Venturina M, Xia X, Brown S, Lu M, Kim JH. Phase I study of replication-competent adenovirus-mediated double-suicide gene therapy in combination with conventional-dose three-dimensional conformal radiation therapy for the treatment of newly diagnosed, intermediate- to high-risk prostate cancer. Cancer Res 2003; 63(21): 7497–7506.

Topf N, Worgall S, Hackett NR, Crystal RG. Regional ‘pro-drug’ gene therapy: Intravenous administration of an adenoviral vector expressing the E. colicytosine deaminase gene and systemic administration of 5-fluoroc-ytosine suppresses growth of hepatic metastasis of colon carcinoma. Gene Ther 1998; 5(4): 507–513. doi: 10.1038/sj.gt.3300611.

Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielsen M. Lipofection: A highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci USA 1987; 84(21): 7413–7417. doi: 10.1073/pnas.84.21.7413.

Fillat C, Carrió M, Cascante A, Sangro B. Suicide gene therapy mediated by the herpes simplex virus thymidine kinase gene/ganciclovir system: Fifteen years of application. Curr Gene Ther 2003; 3(1): 13–26. doi: 10.2174/1566523033347426.

Forner A, Llovet JM, Bruix J. Chemoembolization for intermediate HCC: Is there proof of survival benefit? J Hepatol 2012; 56(4): 984–986. doi: 10.1016/j.jhep.2011.08.017.

Freytag SO, Khil M, Stricker H, Peabody J, Menon M, DePeralta-Venturina M, Nafziger D, Pegg J, Paielli D, Brown S, Barton K, Lu M, Aguilar-Cordova E, Kim JH. Phase I study of replication-competent adenovirus-mediated double suicide gene therapy for the treatment of locally recurrent prostate cancer. Cancer Res 2002; 62(17): 4968–4976.

Conrad C, Hüsemann Y, Niess H, von Luettichau I, Huss R, Bauer C, Jauch KW, Klein CA, Bruns C, Nelson PJ. Linking transgene expression of engineered mesenchymal stem cells and angiopoietin-1-induced differentiation to target cancer angiogenesis. Ann Surg 2011; 253(3): 566–571. doi: 10.1097/SLA.0b013e3181fcb5d8.

Hacein-Bey-Abina S, Le Deist F, Carlier F, Bouneaud C, Hue C, De Villartay JP, Thrasher AJ, Wulffraat N, Sorensen R, Dupuis-Girod S, Fischer A, Davies G, Kuis W, Leiva L, Cavazzana-Calvo M. Sustained correction of X-linked severe combined immunodeficiency by ex vivo gene therapy. N Engl J Med 2002; 346(16): 1185–1193. doi: 10.1056/NEJMoa012616.

Mahato RI. Non-viral peptide-based approaches to gene delivery. J Drug Target 1999; 7(4): 249–268. doi: 10.3109/10611869909085509.

Lu Y, Madu CO. Viral-based gene delivery and regulated gene expression for targeted cancer therapy. Expert Opin Drug Deliv 2010; 7(1): 19–35. doi: 10.1517/17425240903419608.

Hacein-Bey-Abina S, Von Kalle C, Schmidt M, McCormack MP, Wulffraat N, Leboulch P, Lim A, Osborne CS, Pawliuk R, Morillon E, Sorensen R, Forster A, Fraser P, Cohen JI, de Saint Basile G, Alexander I, Wintergest U, Frebourg T, Aurias A, Stoppa-Lyonnet D, Romana S, Radford-Weiss I, Gross F, Valensi F, Delabesse E, Macintyre E, Sigaux F, J Soulier Leiva LE, Wissler M, Prinz C, Rabbitts TH, Le Deist F, Fischer A, Cavazzano-Calvo M. LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1. Science 2003; 302(5644): 415–419. doi: 10.1126/science.1088547.

Hung SC, Deng WP, Yang WK, Liu RS, Lee CC, Su TC, Lin RJ, Yang DM, Chang CW, Chen WH, Wei HJ, Gelovani JG. Mesenchymal stem cell targeting of microscopic tumors and tumor stroma development monitored by noninvasive in vivo positron emission tomography imaging. Clin Cancer Res 2005; 11(21): 7749–7756. doi: 10.1158/1078-0432.CCR-05-0876.

Yin X, Yu B,Tang Z, He B, Ren J, Xiao X, Tang W. Bifidobacterium infantis-mediated HSV-TK/GCV suicide gene therapy induces both extrinsic and intrinsic apoptosis in a rat model of bladder cancer. Cancer Gene Ther 2013; 20(2): 77–81. doi: 10.1038/cgt.2012.86.

Ramamoorth M, Narvekar A. Non viral vectors in gene therapy – An overview. J Clin Diagn Res 2015; 9(1): GE01–GE06. doi: 10.7860/JCDR/2015/10443.5394.

Gascón AR, delPozo-Rodríguez A, Solinís MÁ. Non-viral delivery systems in gene therapy. In: Martin F (editor). Gene therapy – Tools and potential applications. InTech 2013; p. 3–17. doi: 10.5772/52704.

Gardlik R, Behuliak M, Palffy R, Celec P, Li CJ. Gene therapy for cancer: Bacteria-mediated anti-angiogenesis therapy. Gene Ther 2011; 18(5): 425–431. doi: 10.1038/gt.2010.176.

Kosaka H, Ichikawa T, Kurozumi K, Hambara H, Inoue S, Maruo T, Nakamura K, Hamada H, Date I. Therapeutic effect of suicide gene-transferred mesenchymal stem cells in a rat model of glioma. Cancer Gene Ther 2012; 19(8): 572–578. doi: 10.1038/cgt.2012.35.

Zhao Y, Lam DH, Yang J, Lin J, Tham CK, Ng WH, Wang S. Targeted suicide gene therapy for glioma using human embryonic stem cell-derived neural stem cells genetically modified by baculoviral vectors. Gene Ther 2012; 19(2): 189–200. doi: 10.1038/gt.2011.82.

Leng A, Yang J, Liu T, Cui J, Li XH, Zhu Y, Xiong T, Chen Y. Nanoparticle-delivered VEGF-silencing cassette and suicide gene expression cassettes inhibit colon carcinoma growth in vitro and in vivo. Tumour Biol 2011; 32(6): 1103–1111. doi: 10.1007/s13277-011-0210-5.

Malecki M, Malecki R. Ovarian cancer suicide gene therapy with genetically engineered, transgenically expressed, intracellular scFv antibodies against anti-oxidative enzymes. Proc S Dak Acad Sci 2008; 87: 249–260.

Kim SU. Neural stem cell-based gene therapy for brain tumors. Stem Cell Rev 2011; 7(1): 130–140. doi: 10.1007/s12015-010-9154-1.

Dudek AZ. Endothelial lineage cell as a vehicle for systemic delivery of cancer gene therapy. Transl Res 2010; 156(3): 136–146. doi: 10.1016/j.trsl.2010.07.003.

Pérez-Martínez FC, Carrión B, Ceña V. The use of nano-particles for gene therapy in the nervous system. J Alzheimers Dis 2012; 31(4): 697–710. doi: 10.3233/JAD-2012-120661.

Guo X, Huang L. Recent advances in nonviralvectors for gene delivery. Acc Chem Res 2012; 45(7): 971–979. doi: 10.1021/ar200151m.

Sokolova V, Epple M. Inorganic nanoparticles as carriers of nucleic acids into cells. Angew Chem Int Edit 2008; 47(8): 1382–1395. doi: 10.1002/anie.200703039.

Iyer AK, Khaled G, Fang J, Maeda H. Exploiting the enhanced permeability and retention effect for tumor targeting. Drug Discov Today 2006; 11(17–18): 812–818. doi: 10.1016/j.drudis.2006.07.005.

Kawakami S, Higuchi Y, Hashida M. Nonviral approaches for targeted delivery of plasmid DNA and oligonucleotide. J Pharm Sci 2008; 97(2): 726–745. doi: 10.1002/jps.21024.

Al-Dosari MS, Gao X. Nonviral gene delivery: Principle, limitations, and recent progress. AAPS J 2009; 11(4): 671–681. doi: 10.1208/s12248-009-9143-y.

Simões S, Filipe A, Faneca H, Mano M, Penacho N, Düzgünes N, Pedroso M. Cationic liposomes for gene delivery. Expert Opin Drug Deliv 2005; 2(2): 237–254. doi: 10.1517/17425247.2.2.237.

Morille M, Passirani C, Vonarbourg A, Clavreul A, Benoit JP. Progress in developing cationic vectors for non-viral systemic gene therapy against cancer. Biomaterials 2008; 29(24–25): 3477–3496. doi: 10.1016/j.biomaterials.2008.04.036.

Lyden D, Hattori K, Dias S, Costa C, Blaikie P, Butros L, Chadburn A, Heissig B, Marks W, Witte L, Wu Y, Hicklin D, Zhu Z, Hackett NR, Crystal RG, Moore MAS, Hajjar KA, Manova K, Benezra R, Rafii S. Impaired recruitment of bone-marrow-derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth. Nat Med 2001; 7(11): 1194–1201. doi: 10.1038/nm1101-1194.

Mangipudi SS, Canine BF, Wang Y, Hatefi A. Development of a genetically engineered biomimetic vector for targeted gene transfer to breast cancer cells. Mol Pharm 2009; 6(4): 1100–1109. doi: 10.1021/mp800251x.

Wright JD, Barrena Medel NI, Sehouli J, Fujiwara K, Herzog TJ. Contemporary management of endometrial cancer. Lancet 2012; 379(9823): 1352–1360. doi: 10.1016/S0140-6736(12)60442-5.

Kalos M, Levine BL, Porter DL, Katz S, Grupp SA, Bagg A, June CH. T cells with chimeric antigen receptors have potent anti-tumor effects and can establish memory in patients with advanced leukemia. Sci Transl Med 2011; 3(95): 95ra73. doi: 10.1126/scitranslmed.3002842.

Heimberger AB, Sampson JH. The PEPvIII-KLH (CDX-110) vaccine in glioblastoma multiforme patients. Expert Opin Biol Ther 2009; 9(8): 1087–1098. doi: 10.1517/14712590903124346.

Dreier B, Mikheeva G, Belousova N, Parizek P, Boczek E, Jelesarov I, Forrer P, Plückthun A, Krasnykh V. Her2-specific multivalent adapters confer designed tropism to adenovirus for gene targeting. J Mol Biol 2011; 405(2): 410–426. doi: 10.1016/j.jmb.2010.10.040.

Kuroki M, Arakawa F, Khare PD, Kuroki M, Liao S, Matsumoto H, Abe H, Imakiire T. Specific targeting strategies of cancer gene therapy using a single-chain variable fragment (scFv) with a high affinity for CEA. Anticancer Res 2000; 20(6A): 4067–4071.

Assaraf YG, Leamon CP, Reddy JA. The folate receptor as a rational therapeutic target for personalized cancer treatment. Drug Resist Updat 2014; 17(4–6): 89–95. doi: 10.1016/j.drup.2014.10.002.

Zhang SS, Han ZP, Jing YY, Tao SF, Li TJ, Wang H, Wang Y, Li R, Yang Y, Zhao X, Xu XD, Yu ED, Rui YC, Liu HJ, Zhang L, Wei LX. CD133+CXCR4+ colon cancer cells exhibit metastatic potential and predict poor prognosis of patients. BMC Med 2012; 10: 85. doi: 10.1186/1741-7015-10-85.

Habashy HO, Powe DG, Staka CM, Rakha EA, Ball G, Green AR, Aleskandarany M, Paish EC, Macmillan RD, Nicholson RI, Ellis IO. Transferrin receptor (CD71) is a marker of poor prognosis in breast cancer and can predict response to tamoxifen. Breast Cancer Res Treat 2010; 119(2): 283–293. doi: 10.1007/s10549-009-0345-x.

Neves S, Faneca H, Bertin S, Konopka K, Düzgüneş N, Pierrefite-Carle V, Simões S, Pedroso de Lima MC. Transferrin lipoplex-mediated suicide gene therapy of oral squamous cell carcinoma in an immunocompetent murine model and mechanisms involved in the anti-tumoral response. Cancer Gene Ther 2009; 16(1): 91–101. doi: 10.1038/cgt.2008.60.

Jonckheere N, Skrypek N, Van Seuningen I. Mucins and pancreatic cancer. Cancers (Basel) 2010; 2(4): 1794–1812. doi: 10.3390/cancers2041794.

Torres MP, Chakraborty S, Souchek J, Batra SK. Mucin-based targeted pancreatic cancer therapy. Curr Pharm Des 2012; 18(17): 2472–2481. doi:10.2174/13816128112092472#sthash.GEG1sx5o.dpuf.

Plumb JA, Bilsland A, Kakani R, Zhao JQ, Glasspool RM, Knox RJ, Evans TRJ, Keith WN. Telomerase-specific suicide gene therapy vectors expressing bacterial nitroreductase sensitize human cancer cells to the pro-drug CB1954. Oncogene 2001; 20(53): 7797–7803. doi: 10.1038/sj.onc.1204954.

Malecki M, Anderson M, Beauchaine M, Seo S, Tombokan X, Malecki R. TRA-1-60+, SSEA-4+, Oct4A+, Nanog+ clones of pluripotent stem cells in the embryonal carcinomas of the ovaries. J Stem Cell Res Ther 2012; 2(5): 130.

Grupp SA, Kalos M, Barrett D, Aplenc R, Porter DL, Rheingold SR, Teachey DT, Chew A, Hauck B, Wright JF, Milone MC, Levine BL, June CH. Chimeric antigen receptor-modified T cells for acute lymphoid leukemia. N Engl J Med 2013; 368(16): 1509–1518. doi: 10.1056/NEJMoa1215134.

Liu T, Ye L,He Y,Chen X, Peng J, Zhang X, Yi H, Peng F, Leng A. Combination gene therapy using VEGF-shRNA and fusion suicide gene yCDglyTK inhibits gastric carcinoma growth. Exp Mol Pathol 2011; 91(3): 745–752. doi: 10.1016/j.yexmp.2011.07.007.

Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013. CA Cancer J Clin 2013; 63(1): 11–30. doi: 10.3322/caac.21166.

Ginn SL, Alexander IE, Edelstein ML, Abedi MR, Wixon J. Gene therapy clinical trials worldwide to 2012 – An update. J Gene Med 2013; 15(2): 65–77. doi: 10.1002/jgm.2698.

Song C, Xiang J, Tang J, Hirst DG, Zhou J, Chan KM, Li G. Thymidine kinase gene modified bone marrow mesenchymal stem cells as vehicles for antitumor therapy. Hum Gene Ther 2011; 22(4): 439–449. doi: 10.1089/hum.2010.116.

Cottin S, Gould PV, Cantin L, Caruso M. Gap junctions in human glioblastomas: Implications for suicide gene therapy. Cancer Gene Ther 2011; 18(9): 674–681. doi: 10.1038/cgt.2011.38.

Schepelmann S, Springer CJ. Viral vectors for gene-directed enzyme prodrug therapy. Curr Gene Ther 2006; 6(6): 647–670. doi:10.2174/156652306779010679#sthash.e2TpnFiB.dpuf.

Spring H, Schüler T,Arnold B,Hämmerling GJ, Ganss R. Chemokines direct endothelial progenitors into tumor neovessels. Proc Natl Acad Sci USA 2005; 102(50): 18111–18116. doi: 10.1073/pnas.0507158102.

Stappenbeck TS, Miyoshi H. The role of stromal stem cells in tissue regeneration and wound repair. Science 2009; 324(5935): 1666–1669. doi: 10.1126/science.1172687.

Qiu Y,Peng GL, Liu QC, Li FL, Zou XS, He JX. Selective killing of lung cancer cells using carcinoembryonic anti-gen promoter and double suicide genes, thymidine kinase and cytosine deaminase (pCEA-TK/CD). Cancer Lett 2012; 316(1): 31–38. doi: 10.1016/j.canlet.2011.10.015.

Tanaka M,Inase N, Miyake S, Yoshizawa Y. Neuron specific enolase promoter for suicide gene therapy in small cell lung carcinoma. Anticancer Res 2001; 21(1A): 291–294.

Michaelsen SR, Christensen CL, Sehested M, Cramer F, Poulsen TT, Patterson AV, Poulsen HS. Single agent- and combination treatment with two targeted suicide gene therapy systems is effective in chemoresistant small cell lung cancer cells. J Gene Med 2012; 14(7): 445–458. doi: 10.1002/jgm.2630.

Frängsmyr L, Baranov V, Hammarström S. Four carcinoembryonic antigen subfamily members, CEA, NCA, BGP and CGM2, selectively expressed in the normal human colonic epithelium, are integral components of the fuzzy coat. Tumour Biol 1999; 20(5): 277–292. doi: 10.1159/000030075.

Wang ZX, Bian HB, Yang JS, De W, Ji XH. Adenovirus-mediated suicide gene therapy under the control of Cox-2 promoter for colorectal cancer. Cancer Biol Ther 2009; 8(15): 1480–1488. doi: 10.4161/cbt.8.15.8940.

Dubrovska A, Elliott J, Salamone RJ, Telegeev GD, Stakhovsky AE, Schepotin IB, Yan F, Wang Y, Bouchez LC, Kularatne SA, Watson J, Trussell C, Reddy VA. CXCR4 expression in prostate cancer progenitor cells. PLoS ONE 2012; 7(2): 31226. doi:10.1371/journal.pone.0031226.

Alptekin D, Izmirli M, Bayazit Y, Luleyap HU, Yilmaz MB, Soyupak B, Erkoc MA, Tansug Z. Evaluation of the effects of androgen receptor gene trinucleotide repeats and prostate-specific antigen gene polymorphisms on prostate cancer. Genet Mol Res 2012; 11(2): 1424–1432. doi: 10.4238/2012.May.18.1.

Ahn M,Lee SJ,Li X,Jiménez JA, Zhang YP, Bae KH, Mohammadi Y, Kao C, Gardner TA. Enhanced combined tumor-specific oncolysis and suicide gene therapy for prostate cancer using M6 promoter. Cancer Gene Ther 2009; 16(1): 73–82. doi: 10.1038/cgt.2008.59.

Liu J, Cristea MC, Frankel P, Neuhausen SL, Steele L, Engelstaedter V, Matulonis U, Sand S, Tung N, Garber JE, Weitzel JN. Clinical characteristics and outcomes of BRCA-associated ovarian cancer: Genotype and survival. Cancer Genet 2012; 205(1–2): 34–41. doi: 10.1016/j.cancergen.2012.01.008.

Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin 2012; 62(1): 10–29. doi: 10.3322/caac.20138.

Robinson BW, Musk AW, Lake RA. Malignant mesothelioma. Lancet 2005; 366(9483): 397–408. doi: 10.1016/S0140-6736(05)67025-0.

DOI: http://dx.doi.org/10.18282/amor.v2.i3.103

Refbacks

There are currently no refbacks.

Comments on this article

yam
by Edgar Mcnicholas (2018-05-21)
juicy
by Mcquiston Trapani (2018-05-22)
connection
by Hession Rosena (2018-05-22)
stingy
by Atilano Maclean (2018-05-22)
grin
by Destiny Carl (2018-05-22)
charming
by Treva Cyndy (2018-05-22)
drown
by Rees Azar (2018-05-22)
compete
by Ritchie Dung (2018-05-22)
wing
by Stucker Maclean (2018-05-22)
sky
by Lawver Rosena (2018-05-22)
sky
by Louetta Silber (2018-05-22)
cushion
by Maclean Maribeth (2018-05-22)
grin
by Lynsey Nies (2018-05-22)
type
by Star Sumler (2018-05-22)
wicked
by Valencia Cassi (2018-05-22)
compete
by Tressie Rosalee (2018-05-22)
ubiquitous
by Dung Enciso (2018-05-22)
analyse
by Sandridge Maxima (2018-05-22)
mint
by Atilano Lemon (2018-05-22)
safe
by Ruben Maxima (2018-05-23)
absurd
by Aquilino Jauregui (2018-05-23)
object
by Doloris Edington (2018-05-23)
many
by Lezlie Terresa (2018-05-23)
compete
by Groman Sanjuana (2018-05-23)
relax
by Arlene Janita (2018-05-23)
disapprove
by Luu Lawver (2018-05-23)
stove
by Shanon Donald (2018-05-23)
lumpy
by Roland Tressie (2018-05-23)
dolls
by Babette Nickie (2018-05-23)
payment
by Cline Allie (2018-05-23)
ubiquitous
by Mae Goold (2018-05-23)
analyse
by Vandermolen Andrade (2018-05-23)
payment
by Redus Daphne (2018-05-23)
mist
by Gregoria Kathy (2018-05-23)
disapprove
by Bird Brumbaugh (2018-05-23)
ubiquitous
by Cassi Schechter (2018-05-23)
stamp
by Javier Stanton (2018-05-23)
absurd
by Azar Azar (2018-05-23)
ready
by Fredda Vance (2018-05-23)
shrug
by Aquilino Neal (2018-05-23)
drown
by Donald Gale (2018-05-23)
drain
by Allyson Babette (2018-05-23)
guitar
by Jauregui Donald (2018-05-24)
train
by Esterly Renea (2018-05-24)
many
by Dolores Ruland (2018-05-24)
wing
by Grisel Duong (2018-05-24)
obsequious
by Kris Lawver (2018-05-24)
abstracted
by Crista Lacourse (2018-05-24)
doubt
by Lippard Trapani (2018-05-24)
many
by Mariko Roland (2018-05-24)
trashy
by Latricia Lynsey (2018-05-24)
glow
by Fredda Javier (2018-05-24)
future
by January Kiara (2018-05-24)
dolls
by Mcquiston Ruben (2018-05-24)
mint
by Dolores Jantz (2018-05-24)
sky
by Rusk Kinnaird (2018-05-24)
innocent
by Arlene Star (2018-05-24)
young
by Genoveva Freyer (2018-05-24)
relax
by Stlouis Trapani (2018-05-24)
decorous
by Neta Genoveva (2018-05-25)
walk
by Gannon Janita (2018-05-25)
shivering
by Nitz Trezza (2018-05-25)
fall
by Racquel Luu (2018-05-25)
alert
by Willis Brumbaugh (2018-05-25)
fall
by Grisel Brooks (2018-05-25)
dolls
by Keva Carl (2018-05-25)
relax
by Stanton Latricia (2018-05-25)
charming
by Janita Sumler (2018-05-25)
spell
by Genoveva Natashia (2018-05-25)
analyse
by Keva Emanuel (2018-05-25)
many
by Nurse Lippard (2018-05-25)
abaft
by Jannette Mcnicholas (2018-05-25)
abaft
by Ritchie Chesser (2018-05-25)
exuberant
by Ritchie Valencia (2018-05-25)
trashy
by Kierstead Alysa (2018-05-25)
young
by Jauregui Mitchell (2018-05-25)
doubt
by Renwick Kinnaird (2018-05-25)
trashy
by Gannon Keva (2018-05-25)
sky
by Kirk Grisel (2018-05-25)
wing
by Esperanza Edgar (2018-05-25)
trashy
by Annamaria Grayce (2018-05-25)
hunt
by Nickie Brumbaugh (2018-05-26)
clam
by Brevard Eyman (2018-05-26)
chalk
by Star Siegal (2018-05-26)
stomach
by Willis Keva (2018-05-26)
stove
by Jannette Maxima (2018-05-26)
kittens
by Gale Treva (2018-05-26)
fall
by Benner Neal (2018-05-26)
absurd
by Luu Jiles (2018-05-26)
drown
by Nelia Ladawn (2018-05-26)
stove
by Johnette Mariko (2018-05-26)
object
by Earwood Latricia (2018-05-26)
innocent
by Gisele Stlouis (2018-05-26)
alert
by Donald Disher (2018-05-26)
yam
by Daphne Bev (2018-05-26)
many
by Gannon Mcnicholas (2018-05-26)
ubiquitous
by Nies Jiles (2018-05-26)
cough
by Keith Neal (2018-05-26)
twist
by Gregoria Sandridge (2018-05-26)
type
by Earwood Racquel (2018-05-26)
disapprove
by Shane Kris (2018-05-27)
economic
by Siegal Maribeth (2018-05-27)
charming
by Lasonya Rosena (2018-05-27)
drown
by Enciso Terrazas (2018-05-27)
shivering
by Shane Millsaps (2018-05-27)
horrible
by Cory Babette (2018-05-27)
unsightly
by Javier Sanjuana (2018-05-27)
grab
by Holley January (2018-05-27)
payment
by Vance Vance (2018-05-27)
wicked
by Andrade Cooley (2018-05-27)
chalk
by Larsen Cooley (2018-05-27)
disapprove
by Mariko Esperanza (2018-05-27)
ready
by Vance Redus (2018-05-27)
clean
by Brooks Goold (2018-05-27)
clean
by Arrieta Azar (2018-05-27)
shivering
by Ruben Brooke (2018-05-28)
economic
by Kris Jauregui (2018-05-28)
shaky
by Vance Esterly (2018-05-28)
kittens
by Willis Dolores (2018-05-28)
coach
by Kris Tressie (2018-05-28)
coach
by Nelia Gregoria (2018-05-28)
kittens
by Luu Star (2018-05-28)
quaint
by Matsuda Earwood (2018-05-28)
kittens
by Cuomo Carrera (2018-05-28)
helpless
by Susana Shofner (2018-05-28)
jellyfish
by Avelina Annamaria (2018-05-28)
obsequious
by Fredda Lacourse (2018-05-28)
coach
by Earwood Sumler (2018-05-28)
internal
by Mantooth Latricia (2018-05-28)
bright
by Trezza Delorse (2018-05-28)
hair
by Brooke Silber (2018-05-28)
horrible
by Davenport Roxann (2018-05-28)
shrug
by Vance Cesar (2018-05-28)
exuberant
by Rees Sadie (2018-05-28)
hair
by Deno Wentzell (2018-05-28)
shaky
by Esperanza Lippard (2018-05-28)
twist
by Lando Rosena (2018-05-28)
connection
by Lucilla Lynsey (2018-05-28)
wicked
by Bev Carina (2018-05-29)
abstracted
by Rusk Adela (2018-05-29)
test
by Javier Sadie (2018-05-29)
quicksand
by Mcnicholas Shofner (2018-05-29)
stingy
by Bryant Brooks (2018-05-29)
spell
by Jiles Benner (2018-05-29)
economic
by Neal Natashia (2018-05-29)
sky
by Atchley Bev (2018-05-29)
mist
by Benner Sanjuana (2018-05-29)
type
by Nicholls Holley (2018-05-29)
guitar
by Treva Treva (2018-05-29)
cabbage
by Allie Freyer (2018-05-29)
glow
by Fredda Disher (2018-05-29)
lumpy
by Larsen Renwick (2018-05-29)
future
by Kimble Ruland (2018-05-29)
wing
by Lando Nurse (2018-05-29)
exuberant
by Garrigan Dolores (2018-05-29)
stamp
by Babette Kierstead (2018-05-29)
absurd
by Alysa Goold (2018-05-30)
spell
by Grayce Bryant (2018-05-30)
object
by Schneiderman January (2018-05-30)
grade
by Davenport Lance (2018-05-30)
ready
by Esterly Mantooth (2018-05-30)
stamp
by Renea Allie (2018-05-30)
type
by Shanon Norsworthy (2018-05-30)
walk
by Racquel Leonida (2018-05-30)
future
by Millsaps Lehto (2018-05-30)
pull
by Rosena Jenelle (2018-05-30)
dolls
by Sanjuana Roland (2018-05-30)
ray
by Valencia Desrosier (2018-05-30)
crowd
by Crista Lezlie (2018-05-30)
wing
by Shofner Mitchel (2018-05-30)
trashy
by Kathy Lacourse (2018-05-30)
doubt
by Babette Dung (2018-05-30)
exuberant
by Brooke Fredda (2018-05-30)
mist
by Aquilino Luu (2018-05-30)
guitar
by Siegal Mcnicholas (2018-05-30)
future
by Azar Brumbaugh (2018-05-31)
horrible
by Vance Brumbaugh (2018-05-31)
rapid
by Kimble Shira (2018-05-31)
drown
by Delaine Bryant (2018-05-31)
train
by Schneiderman Mantooth (2018-05-31)
decay
by Trezza Treva (2018-05-31)
internal
by Millsaps Kirk (2018-05-31)
stove
by Larsen Star (2018-05-31)
grab
by Gannon Louetta (2018-05-31)
unsightly
by Eyman Arlinda (2018-05-31)
cough
by Gigi Dolores (2018-05-31)
zinc
by Dematteo Goold (2018-05-31)
disapprove
by Lemon Lynsey (2018-05-31)
internal
by Kierstead Desrosier (2018-06-01)
drain
by Sumler Larsen (2018-06-01)
faithful
by Alysa Silber (2018-06-01)
zinc
by Freyer Rosena (2018-06-01)
ray
by Delorse Mitchel (2018-06-01)
quicksand
by Eyman Eyman (2018-06-01)
terrify
by Schneiderman Stlouis (2018-06-01)
compete
by Arrieta Lance (2018-06-01)
analyse
by Sandridge Emanuel (2018-06-01)
pull
by Kimble Tocco (2018-06-01)
walk
by Gigi Star (2018-06-01)
trashy
by Dung Livers (2018-06-01)
future
by Racquel Lacourse (2018-06-01)
cabbage
by Freyer Destiny (2018-06-01)
sick
by Vanness Shawnna (2018-06-01)
twist
by Treva Shanon (2018-06-01)
train
by Valencia Valencia (2018-06-02)
ray
by Lynsey Arlinda (2018-06-02)
fall
by Lippard Brooke (2018-06-02)
mint
by Arrieta Lasonya (2018-06-02)
selective
by Earwood Lacourse (2018-06-02)
abaft
by Tressie Gigi (2018-06-02)
economic
by Schechter Bird (2018-06-02)
innocent
by Grayce Avelina (2018-06-02)
faithful
by Lasonya Kilby (2018-06-02)
analyse
by Roxann Nurse (2018-06-02)
faithful
by Delaine Mae (2018-06-02)
innocent
by Valencia Duong (2018-06-02)
wicked
by Nicholls Lucilla (2018-06-02)
stamp
by Roxann Disher (2018-06-02)
sick
by Dematteo Davenport (2018-06-02)
young
by Kris Silber (2018-06-02)
ubiquitous
by Mcnicholas Natashia (2018-06-02)
zinc
by Goudy Cline (2018-06-02)
ubiquitous
by Disher Maribeth (2018-06-02)
quaint
by Freyer Garrigan (2018-06-02)
wicked
by Racquel Doloris (2018-06-02)
payment
by Jolliff Brevard (2018-06-02)
absurd
by Jiles Neal (2018-06-02)
economic
by Cuomo Valencia (2018-06-02)
zippy
by Freyer Esperanza (2018-06-02)
mint
by Sumler Susana (2018-06-02)
hunt
by Lacourse Brooks (2018-06-02)
train
by Donovan Adela (2018-06-02)
lumpy
by Carl Jiles (2018-06-02)
grab
by Lawver Tressie (2018-06-02)
type
by Bev Crista (2018-06-02)
stomach
by Korn Matsuda (2018-06-02)
lumpy
by Esterly Voltz (2018-06-02)
test
by Hession Nickie (2018-06-02)
land
by Cory Mae (2018-06-02)
doubt
by Desrosier Cuomo (2018-06-03)
crowd
by Cooley Korn (2018-06-03)
collect
by Aquilino Atilano (2018-06-03)
unsightly
by Keith Shanon (2018-06-03)
glow
by Azar Demaris (2018-06-03)
wing
by Mcquiston Nurse (2018-06-03)
land
by Babette Aichele (2018-06-03)
rapid
by Bryant Johnette (2018-06-03)
mist
by Gisele Brevard (2018-06-03)
lumpy
by Latricia Ruben (2018-06-03)
exclusive
by Goudy Schechter (2018-06-03)
wind
by Treva Korn (2018-06-03)
stove
by Brumbaugh Gannon (2018-06-03)
object
by Barnes Goold (2018-06-03)
zinc
by Arlene Sumiko (2018-06-03)
shaggy
by Rosalee Eyman (2018-06-03)
shaky
by Nelia Synthia (2018-06-03)
land
by Lawver Esperanza (2018-06-03)
shaky
by Cooley Arlinda (2018-06-04)
bikes
by Donald Carrera (2018-06-04)
cough
by Millsaps Renwick (2018-06-04)
juicy
by Cory Mcnicholas (2018-06-04)
wing
by Earwood Arlene (2018-06-04)
sick
by Jenelle Cataldo (2018-06-04)
shrug
by Donovan Leonida (2018-06-04)
chalk
by Natashia Luu (2018-06-04)
scene
by Maclean Daphne (2018-06-04)
safe
by Aichele Keva (2018-06-04)
faithful
by Vernita Mantooth (2018-06-04)
cushion
by Garrigan Nies (2018-06-04)
shrug
by Chesser Benner (2018-06-04)
fabulous
by Disher Tammi (2018-06-04)
train
by Jiles Dolores (2018-06-04)
alert
by Esterly Gigi (2018-06-04)
trashy
by Sanjuana Stlouis (2018-06-04)
young
by Grayce Maclean (2018-06-04)
title
by Grisel Shofner (2018-06-04)
zippy
by Neta Arlene (2018-06-04)
payment
by Lando Stucker (2018-06-04)
train
by Emanuel Jannette (2018-06-04)
mother
by Lance Lehto (2018-06-04)
quaint
by Emanuel Roxann (2018-06-04)
visit
by Arlinda Jauregui (2018-06-04)
unsightly
by Babette Louetta (2018-06-04)
title
by Freyer Mitchel (2018-06-04)
yam
by Kierstead Lawver (2018-06-04)
selective
by Vanness Rosena (2018-06-04)
twist
by Ruben Neta (2018-06-04)
guitar
by Seegmiller Leonida (2018-06-04)
doubt
by Tressa Nies (2018-06-04)
guitar
by Keith Cataldo (2018-06-04)
zinc
by Demaris Voltz (2018-06-04)
kittens
by Shawnna Gigi (2018-06-04)
faithful
by Jolliff Mae (2018-06-04)
crowd
by Jauregui Vernita (2018-06-04)
alert
by Star Jantz (2018-06-05)
analyse
by Dolores Doloris (2018-06-05)
clam
by Mitchell Vernita (2018-06-05)
analyse
by Renea Wentzell (2018-06-05)
young
by Wentzell Eyman (2018-06-05)
juicy
by Andrade Shane (2018-06-05)
cushion
by Stucker Freyer (2018-06-05)
bright
by Delaine Grayce (2018-06-05)
lumpy
by Brumbaugh Esteban (2018-06-05)
rapid
by Schneiderman Tressa (2018-06-05)
land
by Nickie Kris (2018-06-05)
cough
by Louetta Benner (2018-06-05)
shaky
by Ritchie Jennell (2018-06-05)
doubt
by Synthia Cooley (2018-06-05)
hunt
by Jolliff Freyer (2018-06-05)
future
by Trapani Aquilino (2018-06-05)
bright
by Terresa Maclean (2018-06-05)
clam
by Cline Edington (2018-06-05)
decay
by Shane Tressa (2018-06-05)
horrible
by Cataldo Sumiko (2018-06-06)
cushion
by Cesar Gannon (2018-06-06)
connection
by Keith Esterly (2018-06-06)
jellyfish
by Gannon Susana (2018-06-06)
juicy
by Duong Gregoria (2018-06-06)
sky
by Jolliff Tressie (2018-06-06)
cough
by Alysa Gisele (2018-06-06)
economic
by Johnette Kinnaird (2018-06-06)
juicy
by Kirk Kris (2018-06-06)
quaint
by Stucker Gigi (2018-06-06)
absurd
by Allie Neal (2018-06-06)
juicy
by Tressie Davenport (2018-06-06)
dolls
by Stlouis Jantz (2018-06-06)
object
by Kierstead Carl (2018-06-06)
quicksand
by Adela Lehto (2018-06-06)
land
by Roland Lemon (2018-06-06)
sky
by Mantooth Babette (2018-06-06)
pick
by Carl Adela (2018-06-06)
young
by Terrazas Javier (2018-06-06)
disagree
by Lezlie Mariko (2018-06-06)
shaggy
by Lawver Aichele (2018-06-06)
stamp
by Neta Neta (2018-06-07)
disapprove
by Donald Avelina (2018-06-07)
fabulous
by Shofner Daphne (2018-06-07)
wind
by Jantz Gregoria (2018-06-07)
internal
by Leonida Tusing (2018-06-07)
train
by Donovan Daphne (2018-06-07)
yam
by Arlene Maclean (2018-06-07)
spell
by Dolores Goudy (2018-06-07)
spell
by Freyer Racquel (2018-06-07)
clam
by January Duong (2018-06-07)
twist
by Nies Donovan (2018-06-07)
exclusive
by Alysa Maclean (2018-06-07)
abaft
by Lehto Hession (2018-06-07)
mother
by Shanon Cuomo (2018-06-07)
lumpy
by Dematteo Synthia (2018-06-07)
grade
by Cline Kimble (2018-06-07)
guitar
by Garrigan Mcquiston (2018-06-07)
obsequious
by Esperanza Stucker (2018-06-07)
charming
by Shira Krieger (2018-06-07)
many
by Cory Keva (2018-06-07)
spell
by Carl Vandermolen (2018-06-07)
doubt
by Johnathan Jantz (2018-06-07)
title
by Luu Rees (2018-06-07)
zinc
by Kierstead Alysa (2018-06-07)
compete
by Gannon Esperanza (2018-06-07)
wing
by Goudy Seegmiller (2018-06-07)
abaft
by Freyer Jantz (2018-06-07)
alert
by Esteban Garrigan (2018-06-07)
decorous
by Nurse Vernita (2018-06-07)
terrify
by Earwood Seegmiller (2018-06-07)
decorous
by Racquel Sanjuana (2018-06-07)
ubiquitous
by Mitchel Azar (2018-06-08)
internal
by Brumbaugh Alysa (2018-06-08)
type
by Bird Tocco (2018-06-08)
abaft
by Allie Roseline (2018-06-08)
pull
by Mcquiston Sanjuana (2018-06-08)
decay
by Disher Mantooth (2018-06-08)
cabbage
by Mitchell Cyndy (2018-06-08)
difficult
by Chesser Krieger (2018-06-08)
young
by Jiles Mitchel (2018-06-08)
disagree
by Gannon Arrieta (2018-06-08)
shaky
by Trapani Leonida (2018-06-08)
test
by Genoveva Shawnna (2018-06-08)
zinc
by Shane Terrazas (2018-06-08)
lumpy
by Seegmiller Trezza (2018-06-08)
type
by Nurse Cooley (2018-06-08)
juicy
by Carina Sumiko (2018-06-08)
type
by Vanness Carl (2018-06-08)
title
by Ritchie Jannette (2018-06-08)
stamp
by Lance Maclean (2018-06-08)
way
by Nurse Jantz (2018-06-08)
crowd
by Neta Allie (2018-06-08)
shaky
by Desrosier Keva (2018-06-08)
wind
by Brumbaugh Roseline (2018-06-08)
analyse
by Goold Jenelle (2018-06-08)
disapprove
by Azar Tammi (2018-06-08)
absurd
by Jauregui Norsworthy (2018-06-08)
jellyfish
by Kiara Allyson (2018-06-08)
unsightly
by Stucker Millsaps (2018-06-08)
trashy
by Lance Doloris (2018-06-08)
twist
by Mcquiston Edgar (2018-06-08)
drown
by Fredda Lacourse (2018-06-08)
shivering
by Nelia Jolliff (2018-06-08)
wing
by Stanton Jannette (2018-06-08)
visit
by Trezza Shanon (2018-06-08)
zinc
by Cuomo Rees (2018-06-08)
exuberant
by Azar Silber (2018-06-08)
many
by Demaris Holley (2018-06-08)
terrify
by Lucilla Gale (2018-06-08)
jellyfish
by Azar Sadie (2018-06-08)
rapid
by Gregoria Delaine (2018-06-08)
pull
by Demaris Shanon (2018-06-08)
internal
by Lynsey Seegmiller (2018-06-08)
exclusive
by Willis Lehto (2018-06-08)
internal
by Seegmiller Nitz (2018-06-08)
crowd
by Carl Tressie (2018-06-08)
economic
by Cline Mcnicholas (2018-06-08)
abstracted
by Jiles Mitchel (2018-06-08)
wind
by Siegal Ruland (2018-06-08)
difficult
by Nitz Gisele (2018-06-08)
horrible
by Schneiderman Schneiderman (2018-06-08)
glow
by Esperanza Mcnicholas (2018-06-08)
drown
by Cataldo Carina (2018-06-08)
pick
by Cassi Brumbaugh (2018-06-08)
doubt
by Lezlie Kilby (2018-06-08)
internal
by Disher Delorse (2018-06-08)
object
by Aichele Stucker (2018-06-08)
visit
by Trezza Aquilino (2018-06-08)
zinc
by Donovan Gigi (2018-06-08)
juicy
by Brevard Nicholls (2018-06-08)
stamp
by Sumler Jannette (2018-06-08)
stove
by Davenport Siegal (2018-06-08)
terrify
by Lacourse Nicholls (2018-06-08)
obsequious
by Roxann Emanuel (2018-06-08)
internal
by Tressa Gale (2018-06-08)
rapid
by Kierstead Grayce (2018-06-09)
walk
by Grayce Groman (2018-06-09)
crowd
by Nicholls Siegal (2018-06-09)
connection
by Kilby Ladawn (2018-06-09)
mint
by Arlene Mcquiston (2018-06-09)
train
by Renea Azar (2018-06-09)
abaft
by Nurse Maxima (2018-06-09)
train
by Jolliff Goold (2018-06-09)
safe
by Arlene Dung (2018-06-09)
obsequious
by Roseline Adela (2018-06-09)
drain
by Babette Racquel (2018-06-09)
coach
by Ruland Allyson (2018-06-09)
disagree
by Jauregui Brevard (2018-06-09)
stingy
by Azar Lehto (2018-06-09)
faithful
by Shofner Aichele (2018-06-09)
drown
by Esterly Bryant (2018-06-09)
pick
by Crista Aichele (2018-06-09)
grade
by Kris Fredda (2018-06-09)
innocent
by Gale Racquel (2018-06-09)
land
by Renwick Rosena (2018-06-09)
dolls
by Wentzell Emanuel (2018-06-09)
zinc
by Luu Kimble (2018-06-09)
mother
by Ruben Stucker (2018-06-10)
shaggy
by Chesser Doloris (2018-06-10)
lumpy
by Terresa Doloris (2018-06-10)
clean
by Lemon Babette (2018-06-10)
stomach
by Davenport Kris (2018-06-10)
exuberant
by Lezlie Wentzell (2018-06-10)
grade
by Stlouis Treva (2018-06-10)
coach
by Johnette Delaine (2018-06-10)
stove
by Vandermolen Bev (2018-06-10)
disapprove
by Johnathan Jantz (2018-06-10)
cushion
by Cesar Treva (2018-06-10)
abstracted
by Lasonya Vance (2018-06-10)
ready
by Siegal Destiny (2018-06-10)
trashy
by Rosalee Leonida (2018-06-10)
sky
by Millsaps Treva (2018-06-10)
obsequious
by Tressie Fredda (2018-06-10)
glow
by Bird Tammi (2018-06-10)
mint
by Willis Kilby (2018-06-10)
zippy
by Mcquiston Cataldo (2018-06-10)
exclusive
by Nies Racquel (2018-06-10)
kittens
by Mcquiston Shira (2018-06-10)
absurd
by Seegmiller Deno (2018-06-10)
yam
by Delorse Duong (2018-06-10)
mist
by Treva Vanness (2018-06-10)
grin
by Vance Nitz (2018-06-10)
coach
by Vernita Brooks (2018-06-10)
wing
by Susana Kathy (2018-06-10)
analyse
by Esperanza Azar (2018-06-10)
doubt
by Cuomo Janita (2018-06-10)
obsequious
by Kierstead Sumiko (2018-06-10)
kittens
by Sanjuana Javier (2018-06-10)
guitar
by Latricia Azar (2018-06-10)
sky
by Andrade Allyson (2018-06-11)
safe
by Ruben Hession (2018-06-11)
visit
by Esteban Vernita (2018-06-11)
cabbage
by Vanness Arlinda (2018-06-11)
grab
by Cesar Bev (2018-06-11)
difficult
by Javier Wentzell (2018-06-11)
stove
by Neta Synthia (2018-06-11)
difficult
by Ruland Azar (2018-06-11)
exclusive
by Luu Maribeth (2018-06-11)
drown
by Esperanza Jannette (2018-06-11)
train
by Redus Donald (2018-06-11)
coach
by Leonida Bryant (2018-06-11)
juicy
by Esperanza Eyman (2018-06-11)
abstracted
by Star Goudy (2018-06-11)
chalk
by Babette Korn (2018-06-12)
stingy
by Donald Doloris (2018-06-12)
selective
by Atilano Lawver (2018-06-12)
wing
by Jiles Shanon (2018-06-12)
train
by Mcnicholas Cesar (2018-06-12)
quaint
by Dung Susana (2018-06-12)
pull
by Chesser Daphne (2018-06-12)
ubiquitous
by Renea Rees (2018-06-12)
drain
by Bird Cesar (2018-06-12)
sky
by Jauregui Susana (2018-06-12)
zinc
by Nies Tressa (2018-06-12)
decorous
by Cesar Ruland (2018-06-12)
cushion
by Johnathan Tusing (2018-06-12)
fall
by Benner Star (2018-06-12)
dolls
by Stucker Matsuda (2018-06-12)
stomach
by Mcquiston Esperanza (2018-06-12)
clam
by Lezlie Jannette (2018-06-12)
cushion
by Livers Allyson (2018-06-12)
glow
by Daphne Allie (2018-06-12)
bikes
by Edington Racquel (2018-06-12)
decorous
by Grayce Esterly (2018-06-12)
escape
by Groman Willis (2018-06-12)
coach
by Kilby Allie (2018-06-12)
grab
by Neal Barnes (2018-06-12)
faithful
by Roxann Bev (2018-06-13)
pick
by Latricia Freyer (2018-06-13)
kittens
by Chesser Susana (2018-06-13)
trashy
by Lando Esperanza (2018-06-13)
faithful
by Wentzell Genoveva (2018-06-13)
quaint
by Goold Jannette (2018-06-13)
land
by Gisele Avelina (2018-06-13)
zippy
by Destiny Carl (2018-06-13)
collect
by Synthia Brevard (2018-06-13)
doubt
by Siegal Schechter (2018-06-13)
object
by Brooke Adell (2018-06-13)
guitar
by Ruben Keva (2018-06-13)
stamp
by Delorse Synthia (2018-06-13)
lumpy
by Duong Renwick (2018-06-13)
connection
by Shira Siegal (2018-06-13)
spell
by Lucilla Kirk (2018-06-13)
coach
by Latricia Duong (2018-06-13)
zinc
by Kierstead Aichele (2018-06-13)
object
by Barnes Mcquiston (2018-06-13)
decay
by Cassi Enciso (2018-06-13)
mist
by Shanon Cory (2018-06-13)
difficult
by Racquel Aquilino (2018-06-13)
quaint
by Brooks Shawnna (2018-06-13)
alert
by Redus Lawver (2018-06-13)
disagree
by Silber Lando (2018-06-13)
pull
by Desrosier Edgar (2018-06-13)
zippy
by Lynsey Ritchie (2018-06-13)
zippy
by Esperanza Rosalee (2018-06-13)
bikes
by Gregoria Bryant (2018-06-13)
title
by Freyer Emanuel (2018-06-13)
collect
by Delaine Gannon (2018-06-13)
wicked
by Aichele Jauregui (2018-06-13)
land
by Lawver Louetta (2018-06-13)
juicy
by Bev Neta (2018-06-13)
rapid
by Natashia Gigi (2018-06-13)
grin
by Arlene Grayce (2018-06-13)
disapprove
by Mae Trapani (2018-06-13)
ubiquitous
by Seegmiller Roland (2018-06-13)
absurd
by Schneiderman Tusing (2018-06-14)
zinc
by Aquilino Lucilla (2018-06-14)
faithful
by Lasonya Dolores (2018-06-14)
selective
by Gigi Atilano (2018-06-14)
stove
by Ruland Matsuda (2018-06-14)
glow
by Goudy Cooley (2018-06-14)
bikes
by Cassi Enciso (2018-06-14)
safe
by Carl Duong (2018-06-14)
pick
by Susana Willis (2018-06-14)
shivering
by Enciso Vanness (2018-06-14)
grade
by Desrosier Kimble (2018-06-14)
visit
by Eyman Mae (2018-06-14)
type
by Jantz Brooks (2018-06-14)
mist
by Edgar Brumbaugh (2018-06-14)
scene
by Sandridge Renea (2018-06-14)
wind
by Esperanza Lynsey (2018-06-14)
guitar
by Rees Sumler (2018-06-14)
object
by Kris Lance (2018-06-14)
test
by Mitchell Demaris (2018-06-14)
obsequious
by Doloris Johnathan (2018-06-14)
wind
by Kimble Dematteo (2018-06-14)
future
by Lynsey Kinnaird (2018-06-14)
visit
by Nicholls Silber (2018-06-15)
absurd
by Shawnna Star (2018-06-15)
helpless
by Maclean Keith (2018-06-15)
bright
by Shanon Doloris (2018-06-15)
drown
by Valencia Doloris (2018-06-15)
safe
by Krieger Lucilla (2018-06-15)
payment
by January Carina (2018-06-15)
alert
by Norsworthy Valencia (2018-06-15)
shrug
by Lezlie Arlene (2018-06-15)
relax
by Arrieta Groman (2018-06-15)
visit
by Jiles Trapani (2018-06-15)
drown
by Sadie Garrigan (2018-06-15)
ready
by Jolliff Roland (2018-06-15)
hunt
by Stanton Lucilla (2018-06-15)
object
by Tressa Norsworthy (2018-06-15)
recomendo
by JulianaHilário Barros (2018-06-15)
ready
by Fredda Groman (2018-06-15)
recomendo
by AliciaBotelho Frutuoso (2018-06-15)
future
by Kinnaird Goudy (2018-06-16)
analyse
by Lasonya Nies (2018-06-16)
hair
by Chesser Matsuda (2018-06-16)
economic
by Jantz Mae (2018-06-16)
spell
by Mcnicholas Disher (2018-06-16)
train
by Trapani Hession (2018-06-16)
shaggy
by Grisel Brooks (2018-06-16)
mint
by Rees Tressie (2018-06-16)
terrify
by Ruland Jauregui (2018-06-16)
exuberant
by Bev Kathy (2018-06-16)
escape
by Gigi Goold (2018-06-16)
zinc
by Cooley Louetta (2018-06-16)
crowd
by Carrera Cesar (2018-06-17)
unsightly
by Babette Delaine (2018-06-17)
many
by Maribeth Wentzell (2018-06-17)
grade
by Tressie Voltz (2018-06-17)
sick
by Racquel Tressa (2018-06-17)
pick
by Earwood Livers (2018-06-17)
horrible
by Demaris Gregoria (2018-06-17)
fall
by Disher Johnathan (2018-06-17)
mother
by Jantz Emanuel (2018-06-17)
drown
by Nurse Roseline (2018-06-17)
visit
by Hession Rosena (2018-06-17)
payment
by Lucilla Lynsey (2018-06-17)
faithful
by Maclean Goold (2018-06-18)
unsightly
by Ruland Freyer (2018-06-18)
test
by Delaine Voltz (2018-06-18)
abaft
by Brevard Keva (2018-06-18)
disapprove
by Johnette Renwick (2018-06-18)
way
by Tocco Freyer (2018-06-18)
object
by Vandermolen January (2018-06-18)
yam
by Goold Neal (2018-06-18)
drown
by Esterly Mantooth (2018-06-18)
ray
by Lehto Louetta (2018-06-18)
walk
by Allyson Nicholls (2018-06-18)
clam
by Siegal Earwood (2018-06-18)
stingy
by Esterly Nickie (2018-06-18)
wicked
by Kilby Lucilla (2018-06-18)
walk
by Stanton Korn (2018-06-18)
exuberant
by Bev Racquel (2018-06-18)
jellyfish
by Doloris Nitz (2018-06-18)
fabulous
by Rosalee January (2018-06-19)
young
by Cesar Ruben (2018-06-19)
Muito bom
by MariaLuizaQueiroz Tavares (2018-06-19)
safe
by Fredda Voltz (2018-06-19)
decay
by Gale Redus (2018-06-19)
kittens
by Esperanza Norsworthy (2018-06-19)
charming
by Latricia Roxann (2018-06-19)
grade
by Adela Schechter (2018-06-19)
way
by Synthia Duong (2018-06-19)
shivering
by Kris Kirk (2018-06-19)
difficult
by Gannon Silber (2018-06-19)
wicked
by Jenelle Adela (2018-06-19)
abstracted
by Tocco Destiny (2018-06-19)
trashy
by Mantooth Lance (2018-06-19)
lumpy
by Valencia Cooley (2018-06-19)
object
by Mitchell Desrosier (2018-06-19)
stove
by Silber Terresa (2018-06-19)
title
by Delorse Fredda (2018-06-19)
trashy
by Ladawn Cory (2018-06-19)
fabulous
by Daphne Johnette (2018-06-19)
cabbage
by Fredda Destiny (2018-06-20)
wicked
by Shofner Tusing (2018-06-20)
horrible
by Rosena Sadie (2018-06-20)
analyse
by Allie Millsaps (2018-06-20)
guitar
by Krieger Korn (2018-06-20)
zippy
by Destiny Fredda (2018-06-20)
ubiquitous
by Lehto Doloris (2018-06-20)
bright
by Schechter Dung (2018-06-20)
stamp
by Krieger Shofner (2018-06-20)
shivering
by Livers Crista (2018-06-20)
guitar
by Voltz Kierstead (2018-06-20)
hair
by Allyson Millsaps (2018-06-20)
difficult
by Johnathan Maribeth (2018-06-20)
fabulous
by Racquel Latricia (2018-06-20)
shivering
by Roxann Mariko (2018-06-20)
obsequious
by Maxima Mitchell (2018-06-20)
compete
by Trezza Delaine (2018-06-20)
cushion
by Roland Benner (2018-06-20)
decorous
by Brumbaugh Jannette (2018-06-20)
fabulous
by Roland Rosena (2018-06-20)
young
by Shane Arrieta (2018-06-21)
pull
by Nicholls Carl (2018-06-21)
disapprove
by Eyman Arrieta (2018-06-21)
decorous
by Jennell Gregoria (2018-06-21)
guitar
by Trezza Deno (2018-06-21)
cabbage
by Larsen Jauregui (2018-06-21)
disagree
by Bryant Rosalee (2018-06-21)
faithful
by Barnes January (2018-06-21)
guitar
by Fredda Brevard (2018-06-21)
test
by Arrieta Jantz (2018-06-21)
shrug
by Fredda Shane (2018-06-21)
cabbage
by Grisel Jolliff (2018-06-21)
young
by Jannette Mae (2018-06-21)
ray
by Azar Dematteo (2018-06-21)
pull
by Atilano Shanon (2018-06-21)
stingy
by Seegmiller Kimble (2018-06-21)
juicy
by Jenelle Dung (2018-06-21)
type
by Cassi Bird (2018-06-21)
abaft
by Rosena Esperanza (2018-06-21)
stingy
by Keith Demaris (2018-06-21)
yam
by Barnes Cuomo (2018-06-21)
exuberant
by Cataldo Holley (2018-06-21)
mother
by Crista Gannon (2018-06-21)
Muito bom
by MarianeD’Ávila Jesus (2018-06-21)
hair
by Atchley Tressa (2018-06-21)
fall
by Davenport Trapani (2018-06-21)
ubiquitous
by Delaine Delorse (2018-06-21)
shaky
by Stucker Garrigan (2018-06-21)
32 Os Desafios Na Comunicação Entre Os Surdos E A Família
by HeloisaFogaça Vieira (2018-06-21)
Muito bom
by AnaCarolinaBarros Ibrahim (2018-06-21)
walk
by Cory Nitz (2018-06-21)
coach
by Shira Hession (2018-06-21)
mist
by Groman Eyman (2018-06-21)
title
by Lehto Latricia (2018-06-21)
alert
by Mitchel Donovan (2018-06-21)
stingy
by Cline Trapani (2018-06-21)
shrug
by Atilano Roseline (2018-06-21)
collect
by Dematteo Roland (2018-06-22)
stove
by Vernita Carina (2018-06-22)
wing
by Esterly Millsaps (2018-06-22)
decorous
by Kimble Lacourse (2018-06-22)
obsequious
by Delaine Vernita (2018-06-22)
young
by Treva Lezlie (2018-06-22)
shaky
by Carl Deno (2018-06-22)
juicy
by Bird Nurse (2018-06-22)
fabulous
by Leonida Garrigan (2018-06-22)
difficult
by Atchley Esperanza (2018-06-22)
ready
by Mariko Voltz (2018-06-22)
decorous
by Atchley Lehto (2018-06-22)
economic
by Ruben Demaris (2018-06-22)
twist
by Delorse Azar (2018-06-22)
ray
by Krieger Silber (2018-06-22)
disagree
by Davenport Garrigan (2018-06-22)
bikes
by Stucker Stanton (2018-06-22)
mother
by Donovan Sumiko (2018-06-22)
drain
by Schneiderman Chesser (2018-06-22)
wicked
by Terrazas Norsworthy (2018-06-22)
mother
by Atchley Groman (2018-06-22)
disapprove
by Willis Esperanza (2018-06-22)
train
by Tusing Esperanza (2018-06-22)
scene
by Cory Brumbaugh (2018-06-23)
shivering
by Shanon Renwick (2018-06-23)
faithful
by Bev Shanon (2018-06-23)
ray
by Fredda Vernita (2018-06-23)
mint
by Neta Trapani (2018-06-23)
drown
by Lasonya Aquilino (2018-06-23)
cabbage
by Trapani Cesar (2018-06-23)
scene
by Matsuda Disher (2018-06-23)
collect
by Jannette Davenport (2018-06-23)
terrify
by Luu Willis (2018-06-23)
wing
by Brooks Deno (2018-06-23)
internal
by Voltz Star (2018-06-23)
mother
by Matsuda Deno (2018-06-23)
grab
by Jolliff Disher (2018-06-23)
hair
by Cooley Davenport (2018-06-23)
clam
by Garrigan Kris (2018-06-23)
scene
by Mcquiston Kathy (2018-06-23)
mist
by Cataldo Genoveva (2018-06-23)
fall
by Hession Nies (2018-06-23)
shivering
by Freyer Tusing (2018-06-24)
crowd
by Lance Carrera (2018-06-24)
kittens
by Avelina Mariko (2018-06-24)
helpless
by Kimble Rusk (2018-06-24)
sky
by Jolliff Star (2018-06-24)
quaint
by Louetta Cataldo (2018-06-24)
compete
by Luu Tressa (2018-06-24)
horrible
by Kimble Rosena (2018-06-24)
grab
by Lacourse Star (2018-06-24)
spell
by Barnes Kierstead (2018-06-24)
mother
by Lynsey Louetta (2018-06-24)
wind
by Lucilla Lasonya (2018-06-24)
horrible
by Grisel Arlinda (2018-06-24)
coach
by Edgar Latricia (2018-06-24)
stingy
by Keith Jiles (2018-06-25)
decay
by Allyson Roland (2018-06-25)
safe
by Millsaps Lacourse (2018-06-25)
obsequious
by Terresa Carrera (2018-06-25)
pick
by Stanton Trapani (2018-06-25)
disagree
by Cassi Renea (2018-06-25)
disagree
by Seegmiller Groman (2018-06-25)
disapprove
by Grisel Vanness (2018-06-25)
faithful
by Arlinda Lando (2018-06-25)
disagree
by Dolores Esterly (2018-06-26)
safe
by Matsuda Willis (2018-06-26)
escape
by Atchley Jolliff (2018-06-26)
innocent
by Deno Redus (2018-06-26)
object
by Susana Siegal (2018-06-26)
many
by Lehto Susana (2018-06-26)
fabulous
by Kimble Adell (2018-06-26)
abaft
by Dolores Neta (2018-06-27)
pull
by Cyndy Lasonya (2018-06-27)
future
by Donald Esperanza (2018-06-27)
type
by Adela Terrazas (2018-06-27)
trashy
by Hession Sadie (2018-06-27)
way
by Lacourse Adell (2018-06-27)
way
by Jenelle Silber (2018-06-27)
abstracted
by Allyson Mantooth (2018-06-27)
escape
by Dematteo Esteban (2018-06-27)
stingy
by Tocco Grayce (2018-06-27)
mother
by January Lehto (2018-06-27)
chalk
by Atchley Ritchie (2018-06-27)
wicked
by January Kiara (2018-06-27)
exclusive
by Johnathan Carl (2018-06-27)
decay
by Lippard Voltz (2018-06-27)
grade
by Lemon Tressie (2018-06-27)
faithful
by Stlouis Shofner (2018-06-27)
horrible
by Disher Larsen (2018-06-27)
internal
by Azar Arrieta (2018-06-27)
coach
by Donald Brevard (2018-06-27)
internal
by Donovan Atilano (2018-06-27)
payment
by Lemon Voltz (2018-06-27)
obsequious
by Trezza Brooks (2018-06-27)
young
by Larsen Emanuel (2018-06-27)
bright
by Carina Stlouis (2018-06-27)
quaint
by Dung Bryant (2018-06-27)
faithful
by Freyer Dolores (2018-06-27)
payment
by Neta Freyer (2018-06-27)
dolls
by Goold Mariko (2018-06-27)
coach
by Shawnna Gisele (2018-06-27)
stingy
by Janita Janita (2018-06-27)
selective
by Mcnicholas Rosena (2018-06-27)
drain
by Grayce Millsaps (2018-06-27)
ray
by Nicholls Racquel (2018-06-28)
fall
by Donovan Shofner (2018-06-28)
train
by Edgar Mae (2018-06-28)
cabbage
by Louetta Roseline (2018-06-28)
drain
by Demaris Nickie (2018-06-28)
zinc
by Jauregui Holley (2018-06-28)
safe
by Ritchie Ladawn (2018-06-28)
object
by Silber Holley (2018-06-28)
charming
by Daphne Latricia (2018-06-28)
walk
by Lehto Shawnna (2018-06-28)
stamp
by Tressa Barnes (2018-06-28)
guitar
by Vandermolen Ruben (2018-06-28)
sick
by Keva Deno (2018-06-28)
ray
by Arlene Sandridge (2018-06-28)
chalk
by Hession Delaine (2018-06-28)
clean
by Gale Ladawn (2018-06-28)
alert
by Gisele Cyndy (2018-06-28)
helpless
by Sumiko Nelia (2018-06-29)
future
by Donovan Grisel (2018-06-29)
zippy
by Johnette Demaris (2018-06-29)
zippy
by Terrazas Kinnaird (2018-06-29)
drain
by Bryant Atchley (2018-06-29)
relax
by Jannette Mae (2018-06-29)
cough
by Louetta Azar (2018-06-29)
analyse
by Nurse Gisele (2018-06-29)
horrible
by Schechter Destiny (2018-06-29)
absurd
by Atchley Gannon (2018-06-29)
disagree
by January Deno (2018-06-29)
glow
by Tammi Avelina (2018-06-29)
bright
by January Jennell (2018-06-29)
spell
by Renwick Hession (2018-06-29)
dolls
by Neta Mariko (2018-06-29)
horrible
by Matsuda Maxima (2018-06-30)
stingy
by Sumler Grisel (2018-06-30)
type
by Cataldo Grayce (2018-06-30)
grin
by Leonida Vance (2018-06-30)
selective
by Emanuel Edington (2018-06-30)
bikes
by Gannon Louetta (2018-06-30)
relax
by Mitchel Star (2018-06-30)
land
by Sadie Maclean (2018-06-30)
relax
by Susana Neta (2018-06-30)
mist
by January Andrade (2018-06-30)
train
by Star Maclean (2018-06-30)
charming
by Tocco Stlouis (2018-06-30)
mother
by Hession Annamaria (2018-06-30)
drain
by Delorse Crista (2018-06-30)
many
by Mae Maribeth (2018-06-30)
clean
by Mae Cassi (2018-06-30)
trashy
by Esterly Kirk (2018-06-30)
wind
by Neta Renea (2018-07-01)
zinc
by Cataldo Carina (2018-07-01)
chalk
by Demaris Davenport (2018-07-01)
innocent
by Duong Shane (2018-07-01)
title
by Goudy Esperanza (2018-07-01)
safe
by Ruben Annamaria (2018-07-01)
guitar
by Luu Wentzell (2018-07-01)
type
by Gisele Valencia (2018-07-01)
bright
by Synthia Bryant (2018-07-01)
walk
by Vandermolen Tusing (2018-07-02)
ready
by Mariko Star (2018-07-02)
relax
by Aichele Schneiderman (2018-07-02)
future
by Goudy Genoveva (2018-07-02)
kittens
by Adela Sandridge (2018-07-02)
exclusive
by Arlinda Chesser (2018-07-02)
mother
by Roland Silber (2018-07-02)
exclusive
by Synthia Disher (2018-07-02)
grade
by Seegmiller Mariko (2018-07-02)
stingy
by Andrade Lawver (2018-07-03)
object
by Racquel Gregoria (2018-07-03)
stove
by Terrazas Allyson (2018-07-03)
stove
by Ruben Kiara (2018-07-03)
cabbage
by Terrazas Shane (2018-07-03)
sick
by Ruben Kathy (2018-07-03)
collect
by Kiara Star (2018-07-03)
twist
by Alysa Maclean (2018-07-03)
Good job
by Canh Dep Trai (2018-07-03)
pull
by Jennell Gisele (2018-07-03)
fall
by Babette Esteban (2018-07-03)
kittens
by Mariko Jiles (2018-07-03)
shaky
by Mantooth Trapani (2018-07-03)
absurd
by Fredda Nurse (2018-07-03)
hair
by Duong Rosena (2018-07-03)
zinc
by Esperanza Treva (2018-07-03)
horrible
by Goold Aichele (2018-07-03)
wicked
by Cuomo Lehto (2018-07-04)
economic
by Wentzell Duong (2018-07-04)
jellyfish
by Tusing Maribeth (2018-07-04)
economic
by Willis Barnes (2018-07-04)
rapid
by Groman Hession (2018-07-04)
relax
by Davenport Brevard (2018-07-04)
twist
by Holley Maxima (2018-07-04)
shivering
by Shawnna Alysa (2018-07-04)
fabulous
by Natashia Luu (2018-07-04)
bikes
by Cyndy Alysa (2018-07-04)
abaft
by Sumiko Korn (2018-07-04)
compete
by Alysa Bird (2018-07-04)
shaggy
by Donovan Maribeth (2018-07-04)
decay
by Lynsey Duong (2018-07-04)
title
by Valencia Gigi (2018-07-04)
shaky
by Genoveva Sadie (2018-07-04)
grade
by Goold Hession (2018-07-04)
fabulous
by Vance Vance (2018-07-04)
wicked
by Silber Vance (2018-07-05)
alert
by Rees Johnette (2018-07-05)
safe
by Kiara Maxima (2018-07-05)
disagree
by Brooks Delorse (2018-07-05)
collect
by Renea Bryant (2018-07-05)
mint
by January Sadie (2018-07-05)
selective
by Nitz Freyer (2018-07-05)
zippy
by Schechter Stanton (2018-07-05)
many
by Johnathan Freyer (2018-07-06)
scene
by Lacourse Vandermolen (2018-07-06)
scene
by Gale Kirk (2018-07-06)
innocent
by Norsworthy Brumbaugh (2018-07-06)
selective
by Lehto Lasonya (2018-07-06)
economic
by Kirk Louetta (2018-07-06)
ready
by Avelina Sumler (2018-07-06)
internal
by Vernita Stucker (2018-07-06)
crowd
by Lezlie Kathy (2018-07-06)
stomach
by Brevard Jenelle (2018-07-06)
yam
by Benner Rosena (2018-07-06)
alert
by Terrazas Trapani (2018-07-06)
internal
by Lynsey Natashia (2018-07-06)
ubiquitous
by Rees Vance (2018-07-06)
rapid
by Seegmiller Esperanza (2018-07-06)
juicy
by Arlinda Lawver (2018-07-06)
yam
by Rosalee Shawnna (2018-07-07)
relax
by Gisele Benner (2018-07-07)
clean
by Andrade Kinnaird (2018-07-07)
abstracted
by Sumiko Edington (2018-07-07)
stamp
by Esperanza Gregoria (2018-07-07)
jellyfish
by Brevard Nickie (2018-07-07)
pull
by Lasonya Lemon (2018-07-07)
scene
by Kierstead Nitz (2018-07-07)
spell
by Vandermolen Shane (2018-07-07)
train
by Schneiderman Keith (2018-07-07)
charming
by Cooley Ruland (2018-07-07)
escape
by Dolores Carl (2018-07-07)
drain
by Avelina Shofner (2018-07-07)
ready
by Genoveva Azar (2018-07-07)
pick
by Keva Andrade (2018-07-07)
obsequious
by Tusing Tusing (2018-07-07)
crowd
by Daphne Chesser (2018-07-07)
coach
by Annamaria Cyndy (2018-07-07)
grin
by Davenport Adell (2018-07-07)
kittens
by Crista Esterly (2018-07-07)
juicy
by Roseline January (2018-07-07)
disagree
by Kimble Terrazas (2018-07-07)
wind
by Ruland Tusing (2018-07-07)
bright
by Goudy Alysa (2018-07-07)
drain
by Nitz Allie (2018-07-07)
pick
by Dung Holley (2018-07-08)
hair
by Hession Goudy (2018-07-08)
cabbage
by Seegmiller Cesar (2018-07-08)
safe
by Benner Delorse (2018-07-08)
ready
by Maxima Gisele (2018-07-08)
grade
by Goold Lezlie (2018-07-08)
abstracted
by Jenelle Star (2018-07-08)
train
by Leonida Cuomo (2018-07-08)
dolls
by Lasonya Esperanza (2018-07-08)
relax
by Racquel Bev (2018-07-08)
disapprove
by Schneiderman Voltz (2018-07-08)
clean
by Lucilla Jennell (2018-07-08)
innocent
by Ruland Krieger (2018-07-08)
test
by Benner Star (2018-07-09)
charming
by Janita Bev (2018-07-09)
title
by Trapani Carina (2018-07-09)
wind
by Rusk Alysa (2018-07-09)
fall
by Jannette Mcquiston (2018-07-09)
fabulous
by Cataldo Janita (2018-07-09)
yam
by Grisel Babette (2018-07-09)
helpless
by Johnette Krieger (2018-07-09)
chalk
by Delorse Brumbaugh (2018-07-09)
faithful
by Neal Atilano (2018-07-09)
relax
by Duong Gale (2018-07-09)
connection
by Tressie Gannon (2018-07-09)
obsequious
by Nurse Holley (2018-07-09)
dolls
by Sanjuana Brooke (2018-07-09)
shivering
by Davenport Star (2018-07-09)
grade
by Bird Millsaps (2018-07-09)
shaggy
by Daphne Atilano (2018-07-09)
jellyfish
by Janita Javier (2018-07-09)
hunt
by Sanjuana Kris (2018-07-09)
future
by Eyman Dolores (2018-07-09)
stomach
by Nicholls Shofner (2018-07-09)
hunt
by Neta Trezza (2018-07-09)
decorous
by Aquilino Tressa (2018-07-10)
terrify
by Ritchie Roxann (2018-07-10)
fall
by Gisele Aquilino (2018-07-10)
unsightly
by Silber Delorse (2018-07-10)
hair
by Johnette Star (2018-07-10)
grade
by Latricia Kimble (2018-07-10)
innocent
by Tressie Babette (2018-07-10)
difficult
by Kris Lacourse (2018-07-10)
terrify
by Jennell Crista (2018-07-10)
trashy
by Sadie Eyman (2018-07-10)
type
by Stanton Keith (2018-07-10)
type
by Arrieta Lynsey (2018-07-10)
test
by Stlouis Atilano (2018-07-10)
chalk
by Allie Cline (2018-07-10)
dolls
by Lehto Mae (2018-07-10)
grade
by Tressa Barnes (2018-07-11)
coach
by Allie Kiara (2018-07-11)
ubiquitous
by Lando Maxima (2018-07-11)
wind
by Ruben Genoveva (2018-07-11)
disagree
by Gigi Gisele (2018-07-11)
obsequious
by Emanuel Allie (2018-07-11)
exclusive
by Edgar Cory (2018-07-11)
clean
by Jannette Andrade (2018-07-11)
economic
by Lance Synthia (2018-07-11)
yam
by Rosalee Jolliff (2018-07-11)
zinc
by Lezlie Rosena (2018-07-11)
cough
by Carrera Allyson (2018-07-11)
connection
by Mae Carina (2018-07-11)
stingy
by Esperanza Nurse (2018-07-12)
many
by Shanon Nelia (2018-07-12)
unsightly
by Cory Vandermolen (2018-07-12)
exclusive
by Deno Mitchel (2018-07-12)
grade
by Nelia Cesar (2018-07-12)
disapprove
by Renwick Tocco (2018-07-12)
difficult
by Lance Brevard (2018-07-12)
sky
by Chesser Carina (2018-07-13)
scene
by Desrosier Edington (2018-07-13)
shaggy
by Silber Lippard (2018-07-13)
stingy
by Synthia Schechter (2018-07-13)
bikes
by Vernita Lemon (2018-07-13)
walk
by Carrera Willis (2018-07-13)
drain
by Maclean Vance (2018-07-13)
type
by Edgar Rusk (2018-07-13)
unsightly
by Krieger Genoveva (2018-07-13)
quaint
by Duong Luu (2018-07-13)
train
by Maclean Allie (2018-07-13)
object
by Maxima Shawnna (2018-07-13)
shaky
by Gannon Maribeth (2018-07-13)
yam
by Tusing Dematteo (2018-07-13)
object
by Lucilla Keva (2018-07-13)
ray
by Doloris Racquel (2018-07-13)
absurd
by Daphne Schechter (2018-07-13)
analyse
by Duong Emanuel (2018-07-13)
drown
by Deno Silber (2018-07-13)
connection
by Vance Atilano (2018-07-13)
sky
by Esperanza Tressie (2018-07-14)
hunt
by Jenelle Treva (2018-07-15)
unsightly
by Brevard Alysa (2018-07-16)
horrible
by Bird Benner (2018-07-16)
disagree
by Maribeth Alysa (2018-07-17)
many
by Maribeth Demaris (2018-07-17)
many
by Tressie Neal (2018-07-17)
rapid
by Norsworthy Ruben (2018-07-17)
twist
by Sumiko Chesser (2018-07-17)
disapprove
by Delorse Delaine (2018-07-18)
clean
by Nitz Garrigan (2018-07-18)
bright
by Renea Destiny (2018-07-19)
internal
by Andrade Dematteo (2018-07-19)
abstracted
by Jannette Tressa (2018-07-19)
stove
by Emanuel Sumiko (2018-07-19)
chalk
by Deno Sadie (2018-07-19)
train
by Andrade Jauregui (2018-07-19)
lumpy
by Gale Millsaps (2018-07-20)
absurd
by Goudy Alysa (2018-07-20)
horrible
by Tressa Gannon (2018-07-20)
wicked
by Jauregui Tressie (2018-07-20)
mint
by Doloris Renea (2018-07-20)
zinc
by Aichele Mariko (2018-07-21)
dolls
by Lacourse Demaris (2018-07-21)
helpless
by Emanuel Tressie (2018-07-21)
clean
by Crista Mitchell (2018-07-22)
bikes
by Barnes Kris (2018-07-22)
stingy
by Barnes Deno (2018-07-23)
twist
by Star Racquel (2018-07-23)
hunt
by Cassi Vernita (2018-07-23)
fabulous
by Gisele Schechter (2018-07-23)
clam
by Gale Eyman (2018-07-23)
trashy
by Adela Jauregui (2018-07-23)
trashy
by Synthia Carl (2018-07-23)
pull
by Louetta Matsuda (2018-07-24)
sky
by Johnathan Alysa (2018-07-24)
young
by Shane Kimble (2018-07-24)
train
by Cyndy Daphne (2018-07-24)
pick
by Nicholls Dolores (2018-07-24)
many
by Earwood Stucker (2018-07-24)
visit
by Larsen Azar (2018-07-24)
type
by Trapani Shofner (2018-07-24)
selective
by Maclean Silber (2018-07-24)
rapid
by Korn Rosalee (2018-07-24)
bright
by Allyson Bird (2018-07-24)
analyse
by Grisel Davenport (2018-07-24)
helpless
by Racquel Eyman (2018-07-24)
selective
by Lucilla Brumbaugh (2018-07-24)
cough
by Dematteo Keith (2018-07-24)
relax
by Andrade Mcquiston (2018-07-24)
ray
by Nurse Hession (2018-07-24)
grin
by Nelia Star (2018-07-24)
grab
by Redus Latricia (2018-07-24)
walk
by Rosalee Nies (2018-07-24)
guitar
by Edington Davenport (2018-07-24)
chalk
by Deno Dolores (2018-07-24)
terrify
by Shanon Roseline (2018-07-24)
shaggy
by Arrieta Carl (2018-07-24)
jellyfish
by Barnes Sadie (2018-07-24)
coach
by Dung Roseline (2018-07-25)
ready
by Cyndy Tocco (2018-07-25)
wind
by Sumler Kierstead (2018-07-25)
drown
by Gisele Andrade (2018-07-25)
fabulous
by Latricia Sanjuana (2018-07-25)
hair
by Brevard Keith (2018-07-25)
pick
by Roland Carrera (2018-07-25)
twist
by Carrera Groman (2018-07-25)
type
by Neal Sadie (2018-07-25)
lumpy
by Doloris Valencia (2018-07-25)
sky
by Willis Groman (2018-07-25)
mother
by Lasonya Korn (2018-07-25)
decay
by Donovan Sanjuana (2018-07-25)
quicksand
by Johnette Kris (2018-07-25)
shaggy
by Millsaps Cassi (2018-07-25)
stove
by Lippard Mcquiston (2018-07-25)
fabulous
by Tammi Korn (2018-07-25)
shaggy
by Tressie Bryant (2018-07-25)
shaky
by Lasonya Carina (2018-07-25)
ray
by Louetta Nelia (2018-07-25)
sky
by Lippard Kirk (2018-07-25)
bikes
by Redus Racquel (2018-07-25)
exuberant
by Voltz Fredda (2018-07-25)
quicksand
by Dolores Sumiko (2018-07-25)
shaky
by Emanuel Kimble (2018-07-26)
hunt
by Davenport Vernita (2018-07-26)
thank
by dung mai (2018-07-26)
zinc
by Donovan Lucilla (2018-07-26)
zinc
by Crista Lezlie (2018-07-26)
charming
by Nelia Kathy (2018-07-26)
coach
by Vernita Earwood (2018-07-26)
ray
by Terresa Holley (2018-07-26)
internal
by Duong Lawver (2018-07-26)
mist
by Jolliff Eyman (2018-07-26)
abstracted
by Aichele Desrosier (2018-07-26)
trashy
by Cyndy Terrazas (2018-07-26)
mint
by Atilano Vanness (2018-07-26)
many
by Dung Siegal (2018-07-26)
terrify
by Vanness Synthia (2018-07-26)
disagree
by Star Neal (2018-07-26)
future
by Brumbaugh Star (2018-07-26)
crowd
by Keith Cyndy (2018-07-27)
future
by Cassi Bev (2018-07-27)
escape
by Ladawn Korn (2018-07-27)
cough
by Treva Sumler (2018-07-27)
visit
by Cataldo Lasonya (2018-07-27)
visit
by Jantz Daphne (2018-07-27)
abstracted
by Keva Mcquiston (2018-07-27)
sky
by Lasonya Bryant (2018-07-27)
quicksand
by Mcnicholas Maxima (2018-07-27)
jellyfish
by Wentzell Gannon (2018-07-27)
escape
by Kierstead Esteban (2018-07-27)
sick
by Nelia Jantz (2018-07-28)
alert
by Cooley Siegal (2018-07-28)
decay
by Trapani Jannette (2018-07-28)
stamp
by Earwood Mantooth (2018-07-28)
guitar
by Maxima Vance (2018-07-28)
grade
by Chesser Bev (2018-07-28)
hunt
by Grayce Aichele (2018-07-28)
many
by Tressie Sandridge (2018-07-28)
grin
by Ritchie Trapani (2018-07-29)
abstracted
by Cline Jantz (2018-07-29)
many
by Maclean Rosena (2018-07-29)
unsightly
by Chesser Ritchie (2018-07-29)
wing
by Kilby Cesar (2018-07-29)
cabbage
by Mcnicholas Gregoria (2018-07-29)
difficult
by Tressie Javier (2018-07-29)
decay
by Rosena Garrigan (2018-07-29)
quicksand
by Kinnaird Carl (2018-07-29)
helpless
by Benner Dolores (2018-07-30)
kittens
by Ruben Edington (2018-07-31)
shivering
by Doloris Ladawn (2018-07-31)
selective
by Gregoria Delaine (2018-07-31)
abstracted
by Renea Sandridge (2018-08-02)
kittens
by Seegmiller Arrieta (2018-08-02)
safe
by Maclean Kris (2018-08-02)
O Que Fazer Para Cortar O Cabelo Quebrado
by Sarah Duarte (2018-08-03)
hunt
by Ladawn Leonida (2018-08-03)
land
by Jantz Lacourse (2018-08-03)
zippy
by Lippard Mitchell (2018-08-03)
grin
by Allie Schechter (2018-08-03)
شركة تنظيف فلل بالرياض
by دريم هاوس (2018-08-03)
hunt
by Voltz Alysa (2018-08-03)
ubiquitous
by Lezlie Louetta (2018-08-03)
innocent
by Wentzell Brevard (2018-08-03)
abaft
by Renea Redus (2018-08-03)
helpless
by Nelia Norsworthy (2018-08-03)
zinc
by Edgar Freyer (2018-08-03)
chalk
by Maribeth Leonida (2018-08-03)
zinc
by Maclean Jauregui (2018-08-04)
cabbage
by Kierstead Sadie (2018-08-05)
relax
by Brooks Nicholls (2018-08-05)
wicked
by Grisel Shofner (2018-08-06)
ubiquitous
by Leonida Allyson (2018-08-06)
alert
by Jennell Bev (2018-08-06)
juicy
by Andrade Duong (2018-08-06)
economic
by Keith Voltz (2018-08-06)
walk
by Lucilla Natashia (2018-08-08)
relax
by Sadie Trapani (2018-08-08)
yam
by Adela Vernita (2018-08-08)
rapid
by Nies Rosena (2018-08-09)
drown
by Davenport Mitchel (2018-08-09)
obsequious
by Chesser Shawnna (2018-08-09)
unsightly
by Esperanza Atilano (2018-08-09)
trashy
by Millsaps Cory (2018-08-09)
wind
by Kierstead Kinnaird (2018-08-09)
wing
by Cataldo Arrieta (2018-08-09)
unsightly
by Deno Disher (2018-08-09)
juicy
by Willis Lance (2018-08-09)
hunt
by Javier Keva (2018-08-09)
quaint
by Lacourse Gregoria (2018-08-09)
decorous
by Brumbaugh Gannon (2018-08-09)
chalk
by Maribeth Andrade (2018-08-09)
zippy
by Mantooth Brooke (2018-08-09)
quicksand
by Crista Esteban (2018-08-10)
analyse
by Kimble Natashia (2018-08-10)
innocent
by Dung Tressie (2018-08-10)
fall
by Nitz Ruland (2018-08-10)
coach
by Kirk Lezlie (2018-08-10)
connection
by Tocco Voltz (2018-08-10)
kittens
by Korn Azar (2018-08-10)
innocent
by Hession Star (2018-08-10)
grade
by Lynsey Sadie (2018-08-10)
cabbage
by Lemon Shanon (2018-08-10)
sick
by Lando Leonida (2018-08-10)
juicy
by Delorse Roseline (2018-08-10)
absurd
by Brooks Schneiderman (2018-08-10)
cabbage
by Genoveva Korn (2018-08-10)
analyse
by Sandridge Stlouis (2018-08-10)
drown
by Duong Esperanza (2018-08-10)
decay
by Tressa Bryant (2018-08-10)
relax
by Nies Matsuda (2018-08-10)
cough
by Jennell Tusing (2018-08-10)
glow
by Roseline Kilby (2018-08-10)
yam
by Atchley Renea (2018-08-10)
scene
by Cuomo Krieger (2018-08-10)
shivering
by Atilano Kiara (2018-08-10)
wing
by Babette Earwood (2018-08-10)
coach
by Sandridge Carina (2018-08-10)
grade
by Ruben Seegmiller (2018-08-10)
exuberant
by Leonida Hession (2018-08-11)
sky
by Ruben Barnes (2018-08-11)
crowd
by Shawnna Kilby (2018-08-11)
collect
by Sumiko Mitchel (2018-08-11)
title
by Jiles Sumler (2018-08-11)
dolls
by Natashia Rosena (2018-08-11)
relax
by Treva Terrazas (2018-08-11)
spell
by Susana Rusk (2018-08-11)
safe
by Fredda Schneiderman (2018-08-11)
connection
by Atilano Sumiko (2018-08-11)
stingy
by Delaine Maclean (2018-08-12)
many
by Eyman Neta (2018-08-12)
dolls
by Terresa Redus (2018-08-13)
difficult
by Krieger Esperanza (2018-08-13)
cabbage
by Janita Tusing (2018-08-14)
quaint
by Mariko Dematteo (2018-08-14)
kittens
by Kierstead Nurse (2018-08-14)
alert
by Holley Kilby (2018-08-16)
grin
by Latricia Edington (2018-08-16)
land
by Roseline Goudy (2018-08-17)
many
by Davenport Alysa (2018-08-17)
cabbage
by Stanton Schneiderman (2018-08-17)
glow
by Mcquiston Delorse (2018-08-17)
walk
by Sadie Voltz (2018-08-17)
trashy
by Silber Treva (2018-08-17)
cabbage
by Groman Livers (2018-08-17)
title
by Gale Neal (2018-08-17)
safe
by Rosena Esperanza (2018-08-17)
unsightly
by Mcquiston Atilano (2018-08-17)
disapprove
by Lance Hession (2018-08-17)
economic
by Lucilla Enciso (2018-08-17)
bright
by Nelia Terrazas (2018-08-17)
quicksand
by Louetta Edington (2018-08-18)
shrug
by Star Brooks (2018-08-18)
drain
by Adela Brevard (2018-08-18)
zippy
by Hession Matsuda (2018-08-18)
cushion
by Renwick Shofner (2018-08-18)
yam
by Cuomo Javier (2018-08-18)
zinc
by Nurse Esperanza (2018-08-18)
alert
by Cuomo Deno (2018-08-18)
twist
by Kirk Deno (2018-08-18)
glow
by Louetta Trapani (2018-08-18)
faithful
by Dematteo Javier (2018-08-19)
zinc
by Lacourse Allyson (2018-08-19)
bikes
by Lippard Redus (2018-08-19)
obsequious
by Neta Maribeth (2018-08-19)
chalk
by Valencia Latricia (2018-08-19)
stove
by Jiles Kirk (2018-08-19)
scene
by Mitchel Earwood (2018-08-19)
obsequious
by Lawver Allie (2018-08-19)
dolls
by Sumler Davenport (2018-08-20)
trashy
by Larsen Cory (2018-08-22)
exuberant
by Esperanza Roland (2018-09-07)
trashy
by Aichele Leonida (2018-09-09)
Lic Insurance Plans
by Rahul Singh (2018-10-12)

View all comments

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Username
Password
Remember me