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Post a Comment The Effect of Thymoquinone on BEAS-2B Cell Viability and TGF-β1 Release
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Thymoquinone, one of the essential oil in the structure of cumin, is used for alternative therapy for many diseases from past to present. It was shown to have anti-carcinogenic and anti-inflammatory effects, as well as positive effects on fibrosis. However, there is no study on the effect of thymoquinone on cancer and fibrosis mechanism in bronchial epithelium cell line BEAS-2B. In our study, the effect of thymoquinone on cell viability and transforming growth factor-beta 1 (TGF-β1) level, which has an important role in the regulation of many biological processes including cancer and fibrosis-associated signal transduction, was evaluated. BEAS-2B cells were exposed to thymoquinone at 0–80 μmol/L concentrations for 24-, 48- and 72-hour durations. Cell viability was evaluated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. TGF-β1 level was determined with enzyme-linked immunosorbent assay (ELISA) method from the collected supernatant. Cell viability was found to be increased at all concentrations and durations (10–80 μmol/L; 24, 48 and 72 h) according to the control group (0 μmol/L; thymoquinone in ethanol) (p < 0.0001). Moreover, thymoquinone was found to increase the level of TGF-β1 only at 80 μmol/L concentration and 24-hour exposure period (0 μmol/L, 53.41 ± 18.44 pgr/ml TGF-β1; 80 μmol/L, 174.5 ± 80.03 pgr/ml TGF-β1). As a result, thymoquinone was found to increase cell proliferation and encourage TGF-β1 release.
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Güllü EB, Gulcan A. Timokinon: Nigella sativa’nın biyoaktif komponenti (Turkish) [Thymoquinone: The bioactive component of Nigella sativa]. Kocatepe Vet J 2013; 6(1).
Pari L, Sankaranarayanan C. Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocinnicotinamide induced diabetic rats. Life Sci 2009; 85(23–26): 830–834. doi: 10.1016/j.lfs.2009.10.021.
Kaseb AO, Chinnakannu K, Chen D, Sivanandam A, Tejwani S, et al. Androgen receptor- and E2F-1-targeted thymoquinone therapy for hormone-refractory prostate cancer. Cancer Res 2007; 67(16): 7782–7788. doi: 10.1158/0008-5472.CAN-07-1483.
Badary OA. Thymoquinone attenuates ifosfamide-induced Fanconi syndrome in rats and enhances its antitumor activity in mice. J Ethnopharmacol 1999; 67(2): 135–142. doi: 10.1016/S0378-8741(98)00242-6.
Kanter M, Coskun O, Budancamanak M. Hepatoprotective effects of Nigella sativa L and Urtica dioica L on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride-treated rats. World J Gastroenterol 2005; 11(42): 6684–6688. doi: 10.3748/wjg.v11.i42.6684.
Badary OA, Abdel-Naim AB, Abdel-Wahab MH, Hamada FM. The influence of thymoquinone on doxorubicin-induced hyperlipidemic nephropathy in rats. Toxicology 2000; 143(3): 219–226. doi: 10.1016/S0300-483X(99)00179-1.
Al-Shabanah OA, Badary OA, Nagi MN, Al-Gharably NM, Al-Rikabi AC, et al. Thymoquinone protects against doxorubicin-induced cardiotoxicity without compromising its antitumor activity. J Exp Clin Cancer Res 1998; 17(2): 193–198.
Salem ML. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol 2005; 5(13–14): 1749–1770. doi: 10.1016/j.intimp.2005.06.008.
Darakhshan S, Pour AB, Colagar AH, Sisakhtnezhad S. Thymoquinone and its therapeutic potentials. Pharmacol Res 2015; 95–96: 138–158. doi: 10.1016/j.phrs.2015.03.011.
Woo CC, Kumar AP, Sethi G, Tan KH. Thymoquinone: Potential cure for inflammatory disorders and cancer.
Biochem Pharmacol 2012; 83(4): 443–451. doi: 10.1016/j.bcp.2011.09.029.
Krstić J, Trivanović D, Mojsilović S, Santibanez JF. Transforming growth factor-beta and oxidative stress
interplay: Implications in tumorigenesis and cancer progression. Oxid Med Cell Longev 2015; 2015: 654594. doi: 10.1155/2015/654594.
Roberts AB, Wakefield LM. The two faces of transforming growth factor beta in carcinogenesis. Proc Natl Acad Sci U S A 2003; 100(15): 8621–8623. doi: 10.1073/pnas.1633291100.
Hughes R, Ward D, Tobin AM, Keegan K, Kirby B. The use of alternative medicine in pediatric patients with atopic dermatitis. Pediatr Dermatol 2007; 24(2): 118–120. doi: 10.1111/j.1525-1470.2007.00355.x.
Chen YF, Chang JS. Complementary and alternative medicine use among patients attending a hospital dermatology clinic in Taiwan. Int J Dermatol 2003; 42(8): 616–621. doi: 10.1046/j.1365-4362.2003.01809.x.
Clarke TC, Black LI, Stussman BJ, Barnes PM, Nahin RL. Trends in the use of complementary health approaches among adults: United States, 2002–2012. Natl Health Stat Report 2015; (79): 1–16.
Loganathan RS, Stover DE, Shi W, Venkatraman E. Prevalence of COPD in women compared to men around the time of diagnosis of primary lung cancer. Chest 2006; 129(5): 1305–1312. doi: 10.1378/chest.129.5.1305.
Young RP, Hopkins RJ, Christmas T, Black PN, Metcalf P, et al. COPD prevalence is increased in lung cancer, independent of age, sex and smoking history. Eur Respir J 2009; 34(2): 380–386. doi: 10.1183/09031936.00144208.
Park J, Kim DS, Shim TS, Lim CM, Koh Y, et al. Lung cancer in patients with idiopathic pulmonary fibrosis. Eur Respir J 2001; 17(6): 1216–1219. doi: 10.1183/09031936.01.99055301.
Kanter M. Thymoquinone attenuates lung injury induced by chronic toluene exposure in rats. Toxicol Ind Health 2011; 27(5): 387–395. doi: 10.1177/0748233710387630.
El-Khouly D, El-Bakly WM, Awad AS, El-Mesallamy HO, El-Demerdash E. Thymoquinone blocks lung injury and fibrosis by attenuating bleomycin-induced oxidative stress and activation of nuclear factor Kappa-B in rats. Toxicology 2012; 302(2–3): 106–113. doi: 10.1016/j.tox.2012.09.001.
Ghazwani M, Zhang Y, Gao X, Fan J, Li J, et al. Antifibrotic effect of thymoquinone on hepatic stellate cells.
Phytomedicine 2014; 21(3): 254–260. doi: 10.1016/j.phymed.2013.09.014.
Bai T, Lian LH, Wu YL, Wan Y, Nan JX. Thymoquinone attenuates liver fibrosis via PI3K and TLR4 signaling pathways in activated hepatic stellate cells. Int Immunopharmacol 2013; 15(2): 275–281. doi: 10.1016/j.intimp.2012.12.020.
Kong LY, Li GP, Yang P, Xi Z. Protective effect of thymoquinone on cholestatic rats with liver injury. Genet Mol Res 2015; 14(4): 12247–12253. doi: 10.4238/2015.October.9.13.
Bai T, Yang Y, Wu YL, Jiang S, Lee JJ, et al. Thymoquinone alleviates thioacetamide-induced hepatic fibrosis and
inflammation by activating LKB1-AMPK signaling pathway in mice. Int Immunopharmacol 2014; 19(2): 351–357. doi:10.1016/j.intimp.2014.02.006.
DOI: http://dx.doi.org/10.18282/amor.v3.i1.170
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